ELISA/assay

Human CCL13/MCP4 ELISA Kit PicoKine®

EK0809

1

Human CCL13/MCP4 ELISA Kit PicoKine®

human-ccl13-mcp4-picokine-trade-elisa-kit-ek0809-boster

Picokine ELISA Kits

CCL13

369

7.8 pg/ml - 500 pg/ml

Human CCL13/MCP4 ELISA Kit PicoKine (96 Tests). Quantitate Human CCL13 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Human CCL13/MCP4 ELISA Kit PicoKine (96 Tests). Quantitate Human CCL13 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

96 wells/kit, with removable strips.

Q99616

Expression system for standard: E.coli; Immunogen sequence: Q24-T98

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

10 ng/tube

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Dilution ratio of 1:1, concentration in serum is around 40pg/ml around 100pg/ml

ELISA

Human

/antibody/ek0809-2-ELISA-human-ccl13-mcp4-picokine-elisa-kit.jpg

Human CCL13/MCP4 PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.004 0.025 0.054 0.116 0.254 0.636 1.469 2.245

5/12/14 3:17

3/2/22 2:54

Human CCL13/MCP4 ELISA Kit Price PicoKine® BosterBio

Human CCL13/MCP4 ELISA Kit, Bosterbio High Sensitivity

Human CCL13/MCP4 ELISA Kit PicoKine (96 Tests). Quantitate Human CCL13 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

97.3

heparin or EDTA

500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.3 pg/ml, 15.6 pg/ml, 7.8 pg/ml

Chemokine (C-C motif) ligand 13 (CCL13) is a small cytokine belonging to the CC chemokine family. Its gene is located on human chromosome 17 within a large cluster of other CC chemokines. CCL13 induces chemotaxis in monocytes, eosinophils, T lymphocytes, and basophils by binding cell surface G-protein linked chemokine receptors such as CCR2, CCR3 and CCR5. Activity of this chemokine has been implicated in allergic reactions such as asthma. CCL13 can be induced by the inflammatory cytokines interleukin-1 and TNF-alpha.

Chemokines,Immunology,Innate Immunity,Macrophage / Inflamm.

C-C motif chemokine 13;CK-beta-10;Monocyte chemoattractant protein 4;Monocyte chemotactic protein 4;MCP-4;NCC-1;Small-inducible cytokine A13;C-C motif chemokine 13, long chain;C-C motif chemokine 13, medium chain;C-C motif chemokine 13, short chain;CCL13;MCP4, NCC1, SCYA13;

C-C motif chemokine 13

10986 MW

Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens.

Secreted.

Widely expressed. Found in small intestine, thymus, colon, lung, trachea, stomach and lymph node. Low levels seen in the pulmonary artery smooth muscle cells.

C-C motif chemokine 13

Belongs to the intercrine beta (chemokine CC) family.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 19 85 256 18 88 273 Standard deviation 1.14 7 13.05 1.26 6.3 19.11 CV (%) 6% 6% 7% 7% 6.4% 7%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 19 19 17 16 17 1.29 7.5% Sample 2 85 88 83 82 84 2.29 2.7% Sample 3 256 236 245 254 247 7.94 3.2% *number of samples for each test n=16.

15-25min

monoclonal antibody from mouse polyclonal antibody from goat

25654294

elisa-kits