ELISA/assay

Mouse IL-27/IL-30 p28 ELISA Kit PicoKine®

EK0800

1

Mouse IL-27/IL-30 p28 ELISA Kit PicoKine®

mouse-il-27-picokine-trade-elisa-kit-ek0800-boster

Picokine ELISA Kits

IL27

369

15.6 pg/ml - 1,000 pg/ml

Mouse IL-27/IL-30 p28 ELISA Kit PicoKine (96 Tests). Quantitate Mouse Il27 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Mouse IL-27/IL-30 p28 ELISA Kit PicoKine (96 Tests). Quantitate Mouse Il27 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

96 wells/kit, with removable strips.

Q8K3I6

Expression system for standard: NS0; Immunogen sequence: F29-S234

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

1 ng/tube

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

ELISA

Mouse

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Mouse IL-27 p28 PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.008 0.041 0.088 0.164 0.298 0.579 1.037 1.859

5/12/14 3:17

3/2/22 2:54

Mouse IL-27/IL-30 p28 ELISA Kit Price PicoKine® BosterBio

Mouse IL-27 p28 ELISA Kit, IL 27/IL 27 p28 ELISA, Bosterbio High Sensitivity

Mouse IL-27/IL-30 p28 ELISA Kit PicoKine (96 Tests). Quantitate Mouse Il27 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

98

heparin or EDTA

1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.2 pg/ml, 15.6 pg/ml

Interleukin-27 (IL-27) is a heterodimeric cytokine belonging to the IL-12 family that is composed of two subunits; Epstein-Barr virus (EBV)-induced gene 3 (EBI3) (also known as IL-27B) and IL27-p28 (known as IL-30). IL-27 is produced by antigen-presenting cells. By genomic sequence analysis, the IL27 p28 gene was mapped to chromosome 16p11. L-27 plays an important function in regulating the activity of B- and T-lymphocytes. The effects of IL-27 are eliciting by its interaction with a specific cell-surface receptor complex composed of two proteins known as IL27R and gp130.

Cancer,Cytokines,Immunology,Innate Immunity,Interleukins,Tumor Immunology

Interleukin-27 subunit alpha;IL-27 subunit alpha;IL-27-A;IL27-A;p28;Il27;Il27a;

Interleukin-27 subunit alpha

26542 MW

Associates with EBI3 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T- helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR which appears to be required but not sufficient for IL-27-mediated signal transduction. IL-27 potentiate the early phase of TH1 response and suppress TH2 and TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21- and ICAM1/ITGAL/ERK-dependent pathways. It also induces STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up- regulation, an event crucial to TH1 cell commitment. It suppresses the expression of GATA3, the inhibitor TH1 cells development. In CD8 T-cells, it activates STATs as well as GZMB. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. Indeed IL27 alone is also able to inhibit the production of IL17 by CD4 and CD8 T-cells. While IL-27 suppressed the development of proinflammatory Th17 cells via STAT1, it inhibits the development of anti-inflammatory inducible regulatory T-cells, iTreg, independently of STAT1. IL-27 has also an effect on cytokine production, it suppresses proinflammatory cytokine production such as IL2, IL4, IL5 and IL6 and activates suppressors of cytokine signaling such as SOCS1 and SOCS3. Apart from suppression of cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T- cells. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines such as IP-10/CXCL10 and MIG/CXCL9.

Secreted . Poorly secreted without coexpression of EBI3.

Expressed in macrophages and dendritic cells.

Interleukin-27 subunit alpha

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 197 420 31 207 394 Standard deviation 1.68 9.65 27.3 1.61 12.83 24.91 CV (%) 4.8% 4.9% 6.5% 5.2% 6.2% 6.5%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 41 39 37 38 2.23 5.8% Sample 2 197 206 189 190 195 6.8 3.4% Sample 3 420 442 424 441 431 9.85 2.2% *number of samples for each test n=16.

15-25min

monoclonal antibody from rat polyclonal antibody from goat

25042924

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