ELISA/assay

Human IL-27 ELISA Kit PicoKine®

EK0799

1

Human IL-27 ELISA Kit PicoKine®

human-il-27-picokine-trade-elisa-kit-ek0799-boster

Picokine ELISA Kits

IL27

420

156 pg/ml - 10,000 pg/ml

Human IL-27 ELISA Kit PicoKine (96 Tests). Quantitate Human IL27 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Human IL-27 ELISA Kit PicoKine (96 Tests). Quantitate Human IL27 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

96 wells/kit, with removable strips.

Q8NEV9

Expression system for standard: NS0; Immunogen sequence: EBI3 (R21-K229) + P28 (F29-P243)

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

10 ng/tube

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit.

ELISA

Human

/antibody/ek0799-2-ELISA-human-il-27-picokine-elisa-kit.jpg

Human IL-27 PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 156 312 625 125000 25000 5000 10000 O.D. 0.075 0.132 0.184 0.277 0.529 1.006 1.497 1.905

5/12/14 3:17

3/2/22 2:54

Human IL-27 ELISA Kit Price PicoKine® BosterBio

Human IL-27 ELISA Kit, IL 27/IL 27 p28 ELISA, Bosterbio High Sensitivity

Human IL-27 ELISA Kit PicoKine (96 Tests). Quantitate Human IL27 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

98

heparin or EDTA

10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 312 pg/ml, 156 pg/ml

Interleukin-27 (IL-27) is a heterodimeric cytokine belonging to the IL-12 family that is composed of two subunits; Epstein-Barr virus (EBV)-induced gene 3 (EBI3) (also known as IL-27B) and IL27-p28 (known as IL-30). IL-27 is produced by antigen-presenting cells.IL-27 plays an important function in regulating the activity of B- and T-lymphocytes. IL-27, a potent inhibitor of T (H)-17 cell development, may be a useful target for treating inflammatory diseases mediated by these cells.

Cancer,Cytokines,Immunology,Innate Immunity,Interleukins,Tumor Immunology

Interleukin-27 subunit alpha;IL-27 subunit alpha;IL-27-A;IL27-A;Interleukin-30;p28;IL27;IL27A, IL30;

Interleukin-27 subunit alpha

27493 MW

Associates with EBI3 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T- helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR which appears to be required but not sufficient for IL-27-mediated signal transduction. IL-27 potentiate the early phase of TH1 response and suppress TH2 and TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21- and ICAM1/ITGAL/ERK-dependent pathways. It also induces STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up- regulation, an event crucial to TH1 cell commitment. It suppresses the expression of GATA3, the inhibitor TH1 cells development. In CD8 T-cells, it activates STATs as well as GZMB. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. Indeed IL27 alone is also able to inhibit the production of IL17 by CD4 and CD8 T-cells. While IL-27 suppressed the development of proinflammatory Th17 cells via STAT1, it inhibits the development of anti-inflammatory inducible regulatory T-cells, iTreg, independently of STAT1. IL-27 has also an effect on cytokine production, it suppresses proinflammatory cytokine production such as IL2, IL4, IL5 and IL6 and activates suppressors of cytokine signaling such as SOCS1 and SOCS3. Apart from suppression of cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T- cells. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines such as IP-10/CXCL10 and MIG/CXCL9. In vein endothelial cells, it induces IRF1/interferon regulatory factor 1 and increase the expression of MHC class II transactivator/CIITA with resulting up-regulation of major histocompatibility complex class II. IL-27 also demonstrates antiviral activity with inhibitory properties on HIV-1 replication.

Secreted . Does not seem to be secreted without coexpression of EBI3.

Expressed in monocytes and in placenta.

Interleukin-27 subunit alpha

Belongs to the IL-6 superfamily.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 266 1556 5164 259 1491 5174 Standard deviation 14.63 716.05 256.2% 16.05 90.95 284.57 CV (%) 5.5% 5% 5% 6.2% 6.1% 5.5%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 266 272 277 241 264 13.83 5.2% Sample 2 1556 1617 1541 1521 1558 35.84 2.3% Sample 3 5164 5696 5356 5370 5396 191.11 3.5% *number of samples for each test n=16.

15-25min

monoclonal antibody from mouse polyclonal antibody from goat

23645091

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