Human IL-1RA/IL1RN ELISA Kit PicoKine¬Æ | Boster EK0782 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human IL-1RA/IL1RN ELISA Kit PicoKine¬Æ

catalog :

EK0782

more info or order :

Boster product webpage

citations: 1

Reference
Carstensen M, Herder C, Kivimaki M, Jokela M, Roden M, Shipley M, et al. Accelerated increase in serum interleukin-1 receptor antagonist starts 6 years before diagnosis of type 2 diabetes: Whitehall II prospective cohort study. Diabetes. 2010;59:1222-7 pubmed publisher

image

image 1 :

Human IL-1RA PicoKine ELISA Kit standard curve

product information

sku :

EK0782

status :

name :

Human IL-1RA/IL1RN ELISA Kit PicoKine¬Æ

url key :

human-il-1ra-picokine-trade-elisa-kit-ek0782-boster

product category :

Picokine ELISA Kits

gene name :

IL1RN

price :

400

assay range :

31.2 pg/ml - 2,000 pg/ml

description :

Human IL-1RA/IL1RN ELISA Kit PicoKine (96 Tests). Quantitate Human IL1RN in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml.

short description :

Human IL-1RA/IL1RN ELISA Kit PicoKine (96 Tests). Quantitate Human IL1RN in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

P18510

immunogen :

Expression system for standard: E.coli; Immunogen sequence: R26-E177

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<2 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

application details :

Dilution ratio of 1:1, concentration in serum is around 800pg/ml

applications :

ELISA

reactivity :

Human

images :

/e/k/ek0782_1.png

image labels :

Human IL-1RA PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.007 0.064 0.128 0.224 0.456 0.842 1.354 1.973

created at :

5/12/14 17:17

updated at :

3/2/22 2:54

meta title :

Human IL-1RA/IL1RN ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Human IL-1RA ELISA Kit, IL 1ra/IL1RN ELISA, Bosterbio High Sensitivity

meta description :

Human IL-1RA/IL1RN ELISA Kit PicoKine (96 Tests). Quantitate Human IL1RN in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml.

rating value :

100

anticoagulant :

heparin or EDTA

assay procedure step 1 :

2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.2 pg/ml

background :

The interleukin-1 receptor antagonist (IL-1RA) is a protein that in humans is encoded by the IL1RN gene. The human IL1RN gene was localized to the long arm of chromosome 2 at band 2q14.2 by fluorescence in situ hybridization.3 IL-1RA is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses.

research category :

Cytokines,Immunology,Innate Immunity,Interleukins

synonyms :

Interleukin-1 receptor antagonist protein;IL-1RN;IL-1ra;IRAP;ICIL-1RA;IL1 inhibitor;Anakinra;IL1RN;IL1F3, IL1RA;

gene full name :

Interleukin-1 receptor antagonist protein

molecular weight :

20055 MW

protein function :

Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.

subcellular localization :

Isoform 1: Secreted.

tissue specificity :

The intracellular form of IL1RN is predominantly expressed in epithelial cells.

protein name :

Interleukin-1 receptor antagonist protein

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 57 296 803 56 318 731 Standard deviation 3.59 20.42 42.55 4.2 27.34 48.97 CV (%) 8.6% 6.9% 5.3% 7.5% 8.6% 6.7%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 57 52 59 58 56 2.69 4.8% Sample 2 296 274 294 294 289 8.98 3.1% Sample 3 803 845 834 932 853 47.86 5.6% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

polyclonal antibody from goat polyclonal antibody from goat

normal range citations :

20185814

template :

elisa-kits

more info or order :

Boster product webpage