Human Galectin-3/LGALS3 ELISA Kit PicoKine¬Æ | Boster EK0764 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human Galectin-3/LGALS3 ELISA Kit PicoKine¬Æ

catalog :

EK0764

more info or order :

Boster product webpage

citations: 1

Reference
Gürses K, Yalçin M, Kocyigit D, Canpinar H, Evranos B, Yorgun H, et al. Effects of persistent atrial fibrillation on serum galectin-3 levels. Am J Cardiol. 2015;115:647-51 pubmed publisher

image

image 1 :

Human Galectin-3/LGALS3 PicoKine ELISA Kit standard curve

product information

sku :

EK0764

status :

name :

Human Galectin-3/LGALS3 ELISA Kit PicoKine¬Æ

url key :

human-galectin-3-lgals3-picokine-trade-elisa-kit-ek0764-boster

product category :

Picokine ELISA Kits

gene name :

LGALS3

price :

369

assay range :

156 pg/ml - 10,000 pg/ml

description :

Human Galectin-3/LGALS3 ELISA Kit PicoKine (96 Tests). Quantitate Human LGALS3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

short description :

Human Galectin-3/LGALS3 ELISA Kit PicoKine (96 Tests). Quantitate Human LGALS3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

P17931

immunogen :

Expression system for standard: E.coli; Immunogen sequence: A2-I250

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<10 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

application details :

Dilution ratio of 1:5, concentration in serum is 4-10ng/ml

applications :

ELISA

reactivity :

Human

images :

/e/k/ek0764_1_1.png

image labels :

Human Galectin-3/LGALS3 PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.029 0.071 0.151 0.265 0.507 0.837 1.315 1.810

created at :

7/11/14 14:17

updated at :

3/2/22 2:54

meta title :

Human Galectin-3/LGALS3 ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Human Galectin-3/LGALS3 ELISA Kit PicoKine, Bosterbio High Sensitivity

meta description :

Human Galectin-3/LGALS3 ELISA Kit PicoKine (96 Tests). Quantitate Human LGALS3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

rating value :

100

anticoagulant :

heparin or EDTA

assay procedure step 1 :

10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 312 pg/ml, 156 pg/ml

background :

Galectin-3 (GAL3), also known as LGALS3, MAC2 or GALBP, is a member of the lectin family, of which 14 mammalian galectins have been identified. Galectin-3 is encoded by a single gene, LGALS3, located on chromosome 14, locus q21‚Äìq22. It is expressed in the nucleus, cytoplasm,mitochondrion, cell surface, and extracellular space. Studies have also shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Galectin-3 is expressed in various tissues and organs, but is significantly absent in normal hepatocytes.

research category :

Cancer,Cell Cycle,Cell Division,Oncoproteins,Tumor Biomarkers

synonyms :

Galectin-3;Gal-3;35 kDa lectin;Carbohydrate-binding protein 35;CBP 35;Galactose-specific lectin 3;Galactoside-binding protein;GALBP;IgE-binding protein;L-31;Laminin-binding protein;Lectin L-29;Mac-2 antigen;LGALS3;MAC2;

gene full name :

Galectin-3

molecular weight :

26152 MW

protein function :

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.

subcellular localization :

Cytoplasm. Nucleus. Secreted. Secreted by a non-classical secretory pathway and associates with the cell surface.

tissue specificity :

A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Expressed in fetal membranes.

protein name :

Galectin-3

sequence similarities :

Contains 1 galectin domain.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 212 1876 4230 205 1951 4307 Standard deviation 16.74 76.91 334.17 19.88 118.70 374.7 CV (%) 7.9% 4.1% 7.9% 9.7% 5.9% 8.7%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 212 196 200 216 206 8.24 4% Sample 2 1876 2001 1983 1897 1939 53.64 2.7% Sample 3 4230 4475 3962 4238 4226 181.56 4.2% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

normal range citations :

25604929

template :

elisa-kits

more info or order :

Boster product webpage