ELISA/assay

Mouse Galectin-1 ELISA Kit PicoKine®

EK0763

1

Mouse Galectin-1 ELISA Kit PicoKine®

mouse-galectin-1-picokine-trade-elisa-kit-ek0763-boster

Picokine ELISA Kits

LGALS1

400

156 pg/ml - 10,000 pg/ml

Mouse Galectin-1 ELISA Kit PicoKine (96 Tests). Quantitate Mouse Lgals1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.

Mouse Galectin-1 ELISA Kit PicoKine (96 Tests). Quantitate Mouse Lgals1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.

96 wells/kit, with removable strips.

P16045

Expression system for standard: E.coli; Immunogen sequence: A2-E135

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<5 pg/ml

10 ng/tube

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Dilution ratio of 1:1, concentration in serum is 1.6-2ng/ml

ELISA

Mouse

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Mouse Galectin-1 PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.033 0.111 0.179 0.301 0.574 0.890 1.600 2.285

7/11/14 14:17

3/2/22 2:54

Mouse Galectin-1 ELISA Kit Price PicoKine® BosterBio

Mouse Galectin-1 ELISA Kit PicoKine, Bosterbio High Sensitivity

Mouse Galectin-1 ELISA Kit PicoKine (96 Tests). Quantitate Mouse Lgals1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.

100

heparin or EDTA

10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 312 pg/ml, 156 pg/ml

Galectin-1 is a protein that in humans is encoded by the LGALS1 gene.The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS1 may act as an autocrine negative growth factor that regulates cell proliferation.Baldini et al. stated that the mouse beta-galactoside-binding protein is an autocrine regulator of cell proliferation with a role in the maintenance of G0 and in the control of G2 traverse. They found that galectin-1 was expressed in a subset of slowly dividing subventricular zone astrocytes, which included the neural stem cells. Intraventricular infusion experiments and phenotypic analysis of knockout mice showed that galectin-1 was an endogenous factor that promoted the proliferation of neural stem cells in adult mouse brain. The standard product used in this kit is recombinant mouse GALECTIN-1, Ala2-Glu135, with the molecular mass of 15KDa.

Cell Biology

Galectin-1;Gal-1;14 kDa lectin;Beta-galactoside-binding lectin L-14-I;Galaptin;Lactose-binding lectin 1;Lectin galactoside-binding soluble 1;S-Lac lectin 1;Lgals1;Gbp;

Galectin-1

14866 MW

May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity. Strong inducer of T-cell apoptosis (By similarity).

Secreted, extracellular space, extracellular matrix .

Galectin-1

Contains 1 galectin domain.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 343 1747 4774 318 1697 4684 Standard deviation 22.29 132.77 300.76 20.67 144.24 304.46 CV (%) 6.5% 7.6% 8.5% 6.5% 8.5% 6.5%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 343 316 342 346 336 12.07 3.5% Sample 2 1747 1764 1871 1625 1751 87.26 4.9% Sample 3 4774 4662 4642 4948 4756 121.46 2.5% *number of samples for each test n=16.

15-25min

monoclonal antibody from rat polyclonal antibody from goat

21424616

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