Human Fetuin A/AHSG ELISA Kit PicoKine¬Æ | Boster EK0757 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human Fetuin A/AHSG ELISA Kit PicoKine¬Æ

catalog :

EK0757

more info or order :

Boster product webpage

citations: 1

Reference
Yin L, Cai W, Chang X, Li J, Su X, Zhu L, et al. Association between fetuin-A levels with insulin resistance and carotid intima-media thickness in patients with new-onset type 2 diabetes mellitus. Biomed Rep. 2014;2:839-842 pubmed

image

image 1 :

Human Fetuin A PicoKine ELISA Kit standard curve

product information

sku :

EK0757

status :

name :

Human Fetuin A/AHSG ELISA Kit PicoKine¬Æ

url key :

human-fetuin-a-picokine-trade-elisa-kit-ek0757-boster

product category :

Picokine ELISA Kits

gene name :

AHSG

price :

400

assay range :

0.78 ng/ml - 50 ng/ml

description :

Human Fetuin A / AHSG ELISA Kit PicoKine (96 Tests). Quantitate Human AHSG in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

short description :

Human Fetuin A / AHSG ELISA Kit PicoKine (96 Tests). Quantitate Human AHSG in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

P02765

immunogen :

Expression system for standard: NS0; Immunogen sequence: A19-V367

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<10 pg/ml

kit components :

50 ng/tube

sample type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

applications :

ELISA

reactivity :

Human

images :

/e/k/ek0757_2.png

image labels :

Human Fetuin A PicoKine ELISA Kit standard curve

sample data :

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.071 0.306 0.396 0.673 1.169 1.585 1.719 2.126

created at :

5/13/14 14:17

updated at :

3/2/22 2:54

meta title :

Human Fetuin A/AHSG ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Human Fetuin A ELISA Kit, AHSG ELISA Kit, Bosterbio High Sensitivity

meta description :

Human Fetuin A / AHSG ELISA Kit PicoKine (96 Tests). Quantitate Human AHSG in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

rating value :

98.1

anticoagulant :

heparin or EDTA

assay procedure step 1 :

50 ng/ml, 25 ng/ml, 12.5 ng/ml, 6.25 ng/ml, 3.12 ng/ml, 1.56 ng/ml, 0.78 ng/ml

background :

Alpha2-HS glycoprotein (AHSG), also known as fetuin-A, is a plasma protein displaying high-affinity interaction with calcium phosphate, by which ectopic vascular calcification is prevented. The AHSG polymorphism is attributable to the hereditary variation of AHSG and phosphate serum levels, which may affect skeletal development and chronic disorders such as vascular calcification.1Human plasma protein alpha 2-HS-glycoprotein (AHSG) is composed of two polypeptide chains, A and B, encoded by a single mRNA. Southern blot analysis of mouse x human somatic cell hybrids has mapped the AHSG gene to human chromosome 3 in the region 3q21----qter. Using a recombinant plasmid containing a 1,538 bp insert spanning the entire AHSG coding region, AHSG was localized to chromosomal bands 3q27----q29 by in situ hybridization.2

research category :

Cardiovascular,Metabolism,Neurogenesis,Neurology Process,Neuroscience,Obesity,Serum Proteins

synonyms :

Alpha-2-HS-glycoprotein;Alpha-2-Z-globulin;Ba-alpha-2-glycoprotein;Fetuin-A;Alpha-2-HS-glycoprotein chain A;Alpha-2-HS-glycoprotein chain B;AHSG;FETUA;PRO2743;

gene full name :

Alpha-2-HS-glycoprotein

molecular weight :

39325 MW

protein function :

Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions.

subcellular localization :

Secreted.

tissue specificity :

Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins.

protein name :

Alpha-2-HS-glycoprotein

sequence similarities :

Belongs to the fetuin family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1646 4628 22095 1708 5021 21195 Standard deviation 126.74 185.82 883.8 143.47 2244 1229.31 CV (%) 7.7% 4% 4% 8.4% 4.5% 5.8%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1646 1726 1766 1528 1666 90.88 5.4% Sample 2 4628 4495 4603 4388 4528 95.27 2.1% Sample 3 22095 25102 22613 25109 23729 1387.88 5.8% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

normal range citations :

25279156

template :

elisa-kits

more info or order :

Boster product webpage