ELISA/assay

Human ErbB-2 ELISA Kit PicoKine®

EK0756

1

Human ErbB-2 ELISA Kit PicoKine®

human-erbb-2-picokine-trade-elisa-kit-ek0756-boster

Picokine ELISA Kits

ERBB2

400

62.5 pg/ml - 4,000 pg/ml

Human ErbB-2 ELISA Kit PicoKine (96 Tests). Quantitate Human ERBB2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml.

Human ErbB-2 ELISA Kit PicoKine (96 Tests). Quantitate Human ERBB2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml.

96 wells/kit, with removable strips.

P04626

Expression system for standard: NS0; Immunogen sequence: T23-T652

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

10 ng/tube

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

ELISA

Human

/antibody/ek0756-2-ELISA-human-erbb-2-picokine-elisa-kit.jpg

Human ErbB-2 PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.036 0.103 0.180 0.306 0.561 1.048 1.657 2.413

5/13/14 14:17

3/2/22 2:54

Human ErbB-2 ELISA Kit Price PicoKine® BosterBio

Human ErbB-2 ELISA Kit, ErbB 2/HER 2 ELISA, Bosterbio High Sensitivity

Human ErbB-2 ELISA Kit PicoKine (96 Tests). Quantitate Human ERBB2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml.

99.4

heparin or EDTA

4,000 pg/ml, 2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml

HER2/neu (also known as ErbB-2) stands for "Human Epidermal growth factor Receptor 2" and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2/neu has also been designated as CD340 (cluster of differentiation 340) and p185. It is encoded by the ERBB2 gene.HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. It is encoded within the genome by HER2/neu, a known proto-oncogene. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. However, ErbB receptors dimerise on ligand binding, and HER2 is the preferential dimerisation partner of other members of the ErbB family.1 The HER2 gene is a proto-oncogene located at the long arm of human chromosome 17 (17q21-q22)2.

Cancer,Cell Type Markers,Growth Factors,Growth Factors/Hormones,Oncoproteins,Protein Phosphorylation,Receptor Tyrosine Kinases,Signal Transduction,Tags & Cell Markers,Tumor Associated,Tumor Biomarkers,Tumor Immunology,Tumor-Associated Antigens,Tyrosine Kinases

Receptor tyrosine-protein kinase erbB-2;2.7.10.1;Metastatic lymph node gene 19 protein;MLN 19;Proto-oncogene Neu;Proto-oncogene c-ErbB-2;Tyrosine kinase-type cell surface receptor HER2;p185erbB2;CD340;ERBB2;HER2, MLN19, NEU, NGL;

Receptor tyrosine-protein kinase erbB-2

137910 MW

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.

Isoform 1: Cell membrane; Single-pass type I membrane protein. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1.

Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.

Receptor tyrosine-protein kinase erbB-2

Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 164 544 1478 167 559 1477 Standard deviation 12.79 38.62 93.11 16.03 40.8 104.86 CV (%) 7.8% 7.1% 7.3%% 9.6% 7.3% 7.1%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 164 178 168 153 165 8.95 5.4% Sample 2 544 528 493 539 526 19.91 3.7% Sample 3 1478 1506 1587 1640 1552 64.34 4.1% *number of samples for each test n=16.

15-25min

polyclonal antibody from goat polyclonal antibody from goat

25791465

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