Human ESM1/Endocan ELISA Kit PicoKine¬Æ | Boster EK0752 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human ESM1/Endocan ELISA Kit PicoKine¬Æ

catalog :

EK0752

more info or order :

Boster product webpage

citations: 5

Reference
Lv Z, Fan Y, Chen H, Zhao D. Endothelial cell-specific molecule-1: a potential serum marker for gastric cancer. Tumour Biol. 2014;35:10497-502 pubmed publisher
Balta I, Balta S, Koryurek O, Demirkol S, Mikhailidis D, Celik T, et al. Serum endocan levels as a marker of disease activity in patients with Behçet disease. J Am Acad Dermatol. 2014;70:291-6 pubmed publisher
Kao S, Chuang C, Tang C, Lin C, Bien M, Yu M, et al. Plasma endothelial cell-specific molecule-1 (ESM-1) in management of community-acquired pneumonia. Clin Chem Lab Med. 2014;52:445-51 pubmed publisher
Mikkelsen M, Shah C, Scherpereel A, Lanken P, Lassalle P, Bellamy S, et al. Lower serum endocan levels are associated with the development of acute lung injury after major trauma. J Crit Care. 2012;27:522.e11-7 pubmed publisher
Bechard D, Meignin V, Scherpereel A, Oudin S, Kervoaze G, Bertheau P, et al. Characterization of the secreted form of endothelial-cell-specific molecule 1 by specific monoclonal antibodies. J Vasc Res. 2000;37:417-25 pubmed

image

image 1 :

Human ESM1/Endocan PicoKine ELISA Kit standard curve

product information

sku :

EK0752

status :

name :

Human ESM1/Endocan ELISA Kit PicoKine¬Æ

url key :

human-esm1-endocan-picokine-trade-elisa-kit-ek0752-boster

product category :

Picokine ELISA Kits

gene name :

ESM1

price :

369

assay range :

31.2 pg/ml - 2,000 pg/ml

description :

Human ESM1/Endocan ELISA Kit PicoKine (96 Tests). Quantitate Human ESM1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

short description :

Human ESM1/Endocan ELISA Kit PicoKine (96 Tests). Quantitate Human ESM1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

Q9NQ30

immunogen :

Expression system for standard: NS0; Immunogen sequence: W20-R184

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<10 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

application details :

Dilution ratio of 1:1, concentration in serum is 200-800pg/ml

applications :

ELISA

reactivity :

Human

images :

/e/k/ek0752.png

image labels :

Human ESM1/Endocan PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.011 0.069 0.114 0.201 0.367 0.708 1.234 1.998

created at :

7/11/14 21:17

updated at :

3/2/22 2:54

meta title :

Human ESM1/Endocan ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Human ESM1/Endocan ELISA Kit, Bosterbio High Sensitivity

meta description :

Human ESM1/Endocan ELISA Kit PicoKine (96 Tests). Quantitate Human ESM1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

rating value :

96.5

anticoagulant :

heparin or EDTA

assay procedure step 1 :

2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.2 pg/ml

background :

Endothelial cell-specific molecule 1, also known as Endocan, is a protein that in humans is encoded by the ESM1 gene. This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. ESM1 has been described as a specific biomarker of tip cells during neoangiogenesis by independent teams. Its expression has been shown to be increase in presence of pro-angiogenic growth factors such as VEGF (vascular endothelial growth factor) or FGF-2 (fibroblast growth factor 2).

research category :

Cell Type Markers,Tags & Cell Markers

synonyms :

Endothelial cell-specific molecule 1;ESM-1;ESM1;

gene full name :

Endothelial cell-specific molecule 1

molecular weight :

20095 MW

protein function :

Involved in angiogenesis; promotes angiogenic sprouting. May have potent implications in lung endothelial cell-leukocyte interactions.

subcellular localization :

Secreted.

tissue specificity :

Expressed in lung, on the vascular capillary network within alveolar walls, and also at lower level in kidney.

protein name :

Endothelial cell-specific molecule 1

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 90 269 832 96 293 860 Standard deviation 7.11 14.79 45.76 8.44 19.33 54.18 CV (%) 7.9% 5.5% 5.5% 8.8% 6.6% 6.3%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 90 83 83 80 84 3.67 4.3% Sample 2 269 235 269 222 248 20.76 8.3% Sample 3 832 815 926 825 849 44.57 5.2% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

polyclonal antibody from goat polyclonal antibody from goat

normal range citations :

24176522, 24108208, 21958978, 11025405, 25056533

template :

elisa-kits

more info or order :

Boster product webpage