Human C-MET/HGFR ELISA Kit PicoKine¬Æ | Boster EK0744 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human C-MET/HGFR ELISA Kit PicoKine¬Æ

catalog :

EK0744

more info or order :

Boster product webpage

citations: 2

Reference
Khoshdelrad N, Salehi Z, Mashayekhi F, Abbasi O, Mirzajani E. Soluble c-Met expression in the peritoneal fluid and serum of patients with different stages of endometriosis. Arch Gynecol Obstet. 2014;289:1107-12 pubmed publisher
Wader K, Fagerli U, Holt R, Børset M, Sundan A, Waage A. Soluble c-Met in serum of patients with multiple myeloma: correlation with clinical parameters. Eur J Haematol. 2011;87:394-9 pubmed publisher

image

image 1 :

Human C-MET/HGFR PicoKine ELISA Kit standard curve

product information

sku :

EK0744

status :

name :

Human C-MET/HGFR ELISA Kit PicoKine¬Æ

url key :

human-c-met-hgfr-picokine-trade-elisa-kit-ek0744-boster

product category :

Picokine ELISA Kits

gene name :

MET

price :

400

assay range :

62.5 pg/ml - 4,000 pg/ml

description :

Human C-MET/HGFR ELISA Kit PicoKine (96 Tests). Quantitate Human MET in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.

short description :

Human C-MET/HGFR ELISA Kit PicoKine (96 Tests). Quantitate Human MET in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

P08581

immunogen :

Expression system for standard: NS0; Immunogen sequence: E25-R307 (alpha) & S308-T932 (beta)

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<5 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

applications :

ELISA

reactivity :

Human

images :

/e/k/ek0744_1.png

image labels :

Human C-MET/HGFR PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.036 0.081 0.118 0.236 0.340 0.552 1.035 1.955

created at :

5/14/14 11:17

updated at :

3/2/22 2:54

meta title :

Human C-MET/HGFR ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Human C-MET/HGFR ELISA Kit, Bosterbio High Sensitivity

meta description :

Human C-MET/HGFR ELISA Kit PicoKine (96 Tests). Quantitate Human MET in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.

rating value :

99.4

anticoagulant :

heparin or EDTA

assay procedure step 1 :

4,000 pg/ml, 2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml

background :

C-Met (MET or MNNG HOS Transforming gene) is a proto-oncogene that encodes a protein known as hepatocyte growth factor receptor (HGFR). MET proto-oncogene has a total length of 125,982 bp, and it is located in the 7q31 locus of chromosome 7. MET is a membrane receptor that is essential for embryonic development and wound healing. Activation of MET triggers mitogenesis, and morphogenesis.

competitor equivalent skus :

sc 8057 sc 514149 sc 514148

research category :

Cancer,Cancer Susceptibility,Epigenetics And Nuclear Signaling,Oncoproteins,Oncoproteins/Suppressors,Protein Phosphorylation,Proto-Oncogenes,Receptor Tyrosine Kinases,Signal Transduction,Transcription,Tyrosine Kinases

synonyms :

Hepatocyte growth factor receptor;HGF receptor;2.7.10.1;HGF/SF receptor;Proto-oncogene c-Met;Scatter factor receptor;SF receptor;Tyrosine-protein kinase Met;MET;

gene full name :

Hepatocyte growth factor receptor

molecular weight :

155541 MW

protein function :

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells.

subcellular localization :

Membrane; Single-pass type I membrane protein.

tissue specificity :

Expressed in normal hepatocytes as well as in epithelial cells lining the stomach, the small and the large intestine. Found also in basal keratinocytes of esophagus and skin. High levels are found in liver, gastrointestinal tract, thyroid and kidney. Also present in the brain.

protein name :

Hepatocyte growth factor receptor

sequence similarities :

Belongs to the protein kinase superfamily. Tyr protein kinase family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 210 601 1336 204 644 1384 Standard deviation 8.61 31.25 84.16 10.81 39.28 95.49 CV (%) 4.1% 5.2% 6.1% 5.3% 6.1% 6.9%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 210 196 181 199 196 10.35 5.2% Sample 2 601 676 644 633 638 26.8 4.2% Sample 3 1336 1534 1527 1377 1443 88.23 6.1% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

normal range citations :

24213016, 21466586

template :

elisa-kits

more info or order :

Boster product webpage