ELISA/assay

Human CXCL5/LIX/ENA-78 ELISA Kit PicoKine®

EK0728

1

Human CXCL5/LIX/ENA-78 ELISA Kit PicoKine®

human-cxcl5-ena-78-picokine-trade-elisa-kit-ek0728-boster

Picokine ELISA Kits

CXCL5

369

31.2 pg/ml - 2,000 pg/ml

Human CXCL5/LIX/ENA-78 ELISA Kit PicoKine (96 Tests). Quantitate Human CXCL5 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml.

Human CXCL5/LIX/ENA-78 ELISA Kit PicoKine (96 Tests). Quantitate Human CXCL5 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml.

96 wells/kit, with removable strips.

P42830

Expression system for standard: E.coli; Immunogen sequence: A37-N114

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

10 ng/tube

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

ELISA

Human

/e/k/ek0728.png

Human CXCL5/ENA-78 PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.075 0.157 0.253 0.423 0.764 1.199 1.728 2.080

7/12/14 18:17

3/2/22 2:54

Human CXCL5/LIX/ENA-78 ELISA Kit Price PicoKine® BosterBio

Human CXCL5/ENA-78 ELISA Kit, ENA 78 ELISA Kit, Bosterbio High Sensitivity

Human CXCL5/LIX/ENA-78 ELISA Kit PicoKine (96 Tests). Quantitate Human CXCL5 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml.

99

heparin, EDTA or citrate

2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.2 pg/ml

C-X-C motif chemokine 5 is a protein that in humans encoded by the CXCL5 gene. The protein encoded by this gene, CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-gamma. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on humanchromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodeling. CXCL5 plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis.

Atherosclerosis,Cardiovascular,Chemokines,Immunology,Innate Immunity,Macrophage / Inflamm.,Vascular Inflammation

C-X-C motif chemokine 5;ENA-78 (1-78);Epithelial-derived neutrophil-activating protein 78;Neutrophil-activating peptide ENA-78;Small-inducible cytokine B5;ENA-78 (8-78);ENA-78 (9-78);CXCL5;ENA78, SCYB5;

C-X-C motif chemokine 5

11972 MW

Involved in neutrophil activation. In vitro, ENA-78 (8- 78) and ENA-78 (9-78) show a threefold higher chemotactic activity for neutrophil granulocytes.

Secreted.

C-X-C motif chemokine 5

Belongs to the intercrine alpha (chemokine CxC) family.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 66 270 964 61 293 970 Standard deviation 2.77 18.09 74.22 3.53 22.26 82.45 CV (%) 4.2% 6.7% 7.7% 5.8% 7.6% 8.5%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 66 77 65 68 69 4.74 6.8% Sample 2 270 257 287 288 275 12.85 4.6% Sample 3 964 912 900 813 897 54.26 6% *number of samples for each test n=16.

15-25min

monoclonal antibody from mouse polyclonal antibody from goat

8083342, 15837658

elisa-kits