Human CD163 / M130 ELISA Kit PicoKine¬Æ | Boster EK0715 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human CD163 / M130 ELISA Kit PicoKine¬Æ

catalog :

EK0715

more info or order :

Boster product webpage

citations: 4

Reference
Wang J, Guo W, Du H, Yu H, Jiang W, Zhu T, et al. Elevated soluble CD163 plasma levels are associated with disease severity in patients with hemorrhagic fever with renal syndrome. PLoS ONE. 2014;9:e112127 pubmed publisher
Kallestrup M, MÃ¸ller H, Tankisi H, Andersen H. Soluble CD163 levels are elevated in cerebrospinal fluid and serum in people with Type 2 diabetes mellitus and are associated with impaired peripheral nerve function. Diabet Med. 2015;32:54-61 pubmed publisher
Rojo Martinez G, Maymó Masip E, Rodriguez M, Solano E, Goday A, Soriguer F, et al. Serum sCD163 levels are associated with type 2 diabetes mellitus and are influenced by coffee and wine consumption: results of the Di@bet.es study. PLoS ONE. 2014;9:e101250 pubmed publisher
Jasiewicz M, Kowal K, Kowal Bielecka O, Knapp M, Skiepko R, Bodzenta Lukaszyk A, et al. Serum levels of CD163 and TWEAK in patients with pulmonary arterial hypertension. Cytokine. 2014;66:40-5 pubmed publisher

image

image 1 :

Human CD163 PicoKine ELISA Kit standard curve

product information

sku :

EK0715

status :

name :

Human CD163 / M130 ELISA Kit PicoKine¬Æ

url key :

human-cd163-picokine-trade-elisa-kit-ek0715-boster

product category :

Picokine ELISA Kits

gene name :

CD163

price :

369

assay range :

1.56 ng/ml - 100 ng/ml

description :

Human CD163 / M130 ELISA Kit PicoKine (96 Tests). Quantitate Human CD163 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 150pg/ml.

short description :

Human CD163 / M130 ELISA Kit PicoKine (96 Tests). Quantitate Human CD163 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 150pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

Q86VB7

immunogen :

Expression system for standard: NS0; Immunogen sequence: G41-S1045

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<150 pg/ml

kit components :

100 ng/tube

sample type :

cell culture supernatants, serum and plasma (heparin).

application details :

Dilution ratio of 1:40, concentration in serum is around 500ng/ml

applications :

ELISA

reactivity :

Human

images :

/e/k/ek0715.png

image labels :

Human CD163 PicoKine ELISA Kit standard curve

sample data :

Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.012 0.046 0.096 0.205 0.449 0.973 1.864 2.469

created at :

7/13/14 1:17

updated at :

3/2/22 2:54

description before attributes :

Featured Product . Fast ELISA kit now available: Boster is proud to announce the newest Picokine Fast ELISA kits. Now you can perform what used to be 4+ hour ELISA assay in under 1.5 hours. Available for Human CD163 / M130 PicoKine Fast ELISA Kit and many others.

meta title :

Human CD163 / M130 ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Human CD163 ELISA Kit, C163A ELISA Kit, Bosterbio High Sensitivity

meta description :

Human CD163 / M130 ELISA Kit PicoKine (96 Tests). Quantitate Human CD163 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 150pg/ml.

rating value :

99.4

anticoagulant :

heparin

assay procedure step 1 :

100 ng/ml, 50 ng/ml, 25 ng/ml, 12.5 ng/ml, 6.25 ng/ml, 3.13 ng/ml, 1.56 ng/ml

background :

CD163 (Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich (SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc.

competitor equivalent skus :

sc 33560 sc 58965 sc 33715 sc 18796 sc 33559 sc 20066 sc 18794

research category :

Atherosclerosis,Cardiovascular,Cell Type Markers,Immunology,Innate Immunity,Macrophage / Inflamm.,Vascular Inflammation

synonyms :

Scavenger receptor cysteine-rich type 1 protein M130;Hemoglobin scavenger receptor;CD163;Soluble CD163;sCD163;CD163;M130;

gene full name :

Scavenger receptor cysteine-rich type 1 protein M130

molecular weight :

125451 MW

protein function :

Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.

subcellular localization :

Soluble CD163: Secreted.

tissue specificity :

Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood.

protein name :

Scavenger receptor cysteine-rich type 1 protein M130

sequence similarities :

Contains 9 SRCR domains.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 3520 11456 44674 3368 11294 48653 Standard deviation 144.32 526.97 3037.82 195.34 632.46 3357.05 CV (%) 4.1% 4.6% 6.8% 5.8% 5.6% 6.9%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 3520 3403 3222 3050 3298 178.59 5.4% Sample 2 11456 11119 13238 12078 11972 807.44 6.7% Sample 3 44674 42932 42302 44756 43666 1072.77 2.4% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

normal range citations :

24548423, 25392926, 25156085, 24978196

template :

elisa-kits

more info or order :

Boster product webpage