Human IL7R/CD127 ELISA Kit PicoKine¬Æ | Boster EK0713 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human IL7R/CD127 ELISA Kit PicoKine¬Æ

catalog :

EK0713

more info or order :

Boster product webpage

citations: 1

Reference
Janot Sardet C, Assouline B, Cheynier R, Morre M, Beq S. A validated assay to measure soluble IL-7 receptor shows minimal impact of IL-7 treatment. J Immunol Methods. 2010;353:115-23 pubmed publisher

image

image 1 :

Human IL7R/CD127 PicoKine ELISA Kit standard curve

product information

sku :

EK0713

status :

name :

Human IL7R/CD127 ELISA Kit PicoKine¬Æ

url key :

human-il7r-cd127-picokine-trade-elisa-kit-ek0713-boster

product category :

Picokine ELISA Kits

gene name :

IL7R

price :

369

assay range :

312 pg/ml - 20,000 pg/ml

description :

Human IL7R/CD127 ELISA Kit PicoKine (96 Tests). Quantitate Human IL7R in cell culture supernatants and serum. Sensitivity: 10pg/ml.

short description :

Human IL7R/CD127 ELISA Kit PicoKine (96 Tests). Quantitate Human IL7R in cell culture supernatants and serum. Sensitivity: 10pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

P16871

immunogen :

Expression system for standard: NS0; Immunogen sequence: E21-I262

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<10 pg/ml

kit components :

20 ng/tube

sample type :

cell culture supernatants and serum.

application details :

Dilution ratio of 1:1, concentration in serum is around 6ng/ml

applications :

ELISA

reactivity :

Human

images :

/e/k/ek0713_1.png

image labels :

Human IL7R/CD127 PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.039 0.235 0.412 0.568 1.021 1.617 1.910 2.168

created at :

5/15/14 1:17

updated at :

3/2/22 2:54

meta title :

Human IL7R/CD127 ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Human IL7R/CD127 ELISA Kit, Bosterbio High Sensitivity

meta description :

Human IL7R/CD127 ELISA Kit PicoKine (96 Tests). Quantitate Human IL7R in cell culture supernatants and serum. Sensitivity: 10pg/ml.

rating value :

100

assay procedure step 1 :

20,000 pg/ml, 10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 312 pg/ml

background :

The interleukin-7 receptor (IL7R) is a protein found on the surface of cells. It is mapped to 5p13.2. The protein encoded by this gene is a receptor for interleukine 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. IL7R has been shown to play a critical role in the development of immune cells called lymphocytes-specifically in a process known as V (D)J recombination. This protein is also found to control the accessibility of a region of the genome that contains the T-cell receptor gamma gene, by STAT5 and histone acetylation. What's more, IL7R antagonism is efficacious in treatment of EAE through its effects on Th17 cells and is a potential treatment for MS.

research category :

Cytokines,Hematopoietic Progenitors,Immunology,Innate Immunity,Interleukins,Lymphoid,Myeloid,Stem Cells,T Lymphocytic Lineage

synonyms :

Interleukin-7 receptor subunit alpha;IL-7 receptor subunit alpha;IL-7R subunit alpha;IL-7R-alpha;IL-7RA;CDw127;CD127;IL7R;

gene full name :

Interleukin-7 receptor subunit alpha

molecular weight :

51581 MW

protein function :

Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP).

subcellular localization :

Isoform 1: Cell membrane; Single-pass type I membrane protein.

protein name :

Interleukin-7 receptor subunit alpha

sequence similarities :

Belongs to the type I cytokine receptor family. Type 4 subfamily.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 410 2617 9061 443 2807 9233 Standard deviation 25.42 120.38 516.47 33.22 140.35 627.84 CV (%) 6.2% 4.6% 5.7% 7.5% 5% 6.8%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 410 395 383 426 403 16.13 4% Sample 2 2617 2389 2451 2237 2423 136.17 5.6% Sample 3 9061 9810 8729 10576 9544 712.96 7.4% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

polyclonal antibody from goat polyclonal antibody from goat

normal range citations :

20035760

template :

elisa-kits

more info or order :

Boster product webpage