ELISA/assay

Human B7-1/CD80 ELISA Kit PicoKine®

EK0707

1

Human B7-1/CD80 ELISA Kit PicoKine®

human-b7-1-cd80-picokine-trade-elisa-kit-ek0707-boster

Picokine ELISA Kits

CD80

369

62.5 pg/ml - 4,000 pg/ml

Human B7-1/CD80 ELISA Kit PicoKine (96 Tests). Quantitate Human CD80 in cell culture supernatants and serum. Sensitivity: 10pg/ml.

Human B7-1/CD80 ELISA Kit PicoKine (96 Tests). Quantitate Human CD80 in cell culture supernatants and serum. Sensitivity: 10pg/ml.

96 wells/kit, with removable strips.

P33681

Expression system for standard: NS0; Immunogen sequence: V35-N242

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

10 ng/tube

cell culture supernatants and serum.

Dilution ratio of 1:1, concentration in serum is around 10pg/ml

ELISA

Human

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Human B7-1/CD80 PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.008 0.052 0.097 0.236 0.508 0.897 1.220 2.123

5/15/14 15:17

3/2/22 2:54

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Human B7-1/CD80 ELISA Kit Price PicoKine® BosterBio

Human B7-1/CD80 ELISA Kit, B7 1, CD80 ELISA, Bosterbio High Sensitivity

Human B7-1/CD80 ELISA Kit PicoKine (96 Tests). Quantitate Human CD80 in cell culture supernatants and serum. Sensitivity: 10pg/ml.

100

4,000 pg/ml, 2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml

The protein CD80 (Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7-1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain. The CD80 and CD86 genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.

Adaptive Immunity,B Cells,Hematopoietic Progenitors,Immunology,Lymphoid,Myeloid,Stem Cells

T-lymphocyte activation antigen CD80;Activation B7-1 antigen;BB1;CTLA-4 counter-receptor B7.1;B7;CD80;CD80;CD28LG, CD28LG1, LAB7;

T-lymphocyte activation antigen CD80

33048 MW

Involved in the costimulatory signal essential for T- lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation.

Membrane; Single-pass type I membrane protein.

Expressed on activated B-cells, macrophages and dendritic cells.

T-lymphocyte activation antigen CD80

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 162 445 1656 163 425 1791 Standard deviation 7.29 34.71 69.55 7.98 34 100.29 CV (%) 4.5% 7.8% 4.2% 4.9% 8% 5.6%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 162 138 164 139 150 12.27 8.1% Sample 2 445 495 515 457 478 28.23 5.9% Sample 3 1656 1667 1402 1560 1571 106.21 6.7% *number of samples for each test n=16.

15-25min

monoclonal antibody from mouse monoclonal antibody from mouse

25142334

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