Mouse CD14 ELISA Kit PicoKine® | Boster EK0695 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse CD14 ELISA Kit PicoKine®

catalog :

EK0695

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK0695

Product Name :

Mouse CD14 ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Mouse CD14 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cd14 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Reactivity :

Mouse

Application :

ELISA

Gene Name :

CD14

Uniprot ID :

P10810

Immunogen :

Expression system for standard: NS0; Immunogen sequence: A18-P345

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

0.78 ng/ml - 50 ng/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

monoclonal antibody from rat, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Monocyte differentiation antigen CD14

Synonyms :

Monocyte differentiation antigen CD14;Myeloid cell-specific leucine-rich glycoprotein;CD14;Cd14;

Protein Name :

Monocyte differentiation antigen CD14

Molecular Weight :

39204 MW

Protein Function :

In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules (By similarity).

Subcellular Localization :

Cell membrane; Lipid-anchor, GPI-anchor.

Background :

CD14, Cluster of differentiation 14, ingle-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. By in situ hybridization and study of somatic cell hybrid DNA that the gene is located at bands 5q23-q31. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.

Research Category :

Cell Type Markers, Hematopoietic Progenitors, Immunology, Innate Immunity, Mesenchymal Stem Cells, Myeloid, Stem Cells, Tlr Signaling