Human CD14 ELISA Kit PicoKine® | Boster EK0694 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human CD14 ELISA Kit PicoKine®

catalog :

EK0694

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK0694

Product Name :

Human CD14 ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Human CD14 ELISA Kit PicoKine® (96 Tests). Quantitate Human CD14 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 50pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

CD14

Uniprot ID :

P08571

Immunogen :

Expression system for standard: CHO; Immunogen sequence: T20-C352

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

156 pg/ml - 10,000 pg/ml

Sensitivity :

<50 pg/ml

Sample Type :

cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Capture/Detection Antibodies :

A monoclonal antibody from mouse, a biotinylated detection polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

CD14 molecule

Synonyms :

Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; CD14

Protein Function :

Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and

Subcellular Localization :

Secreted, Cell membrane, GPI-anchor, Golgi apparatus, Membrane raft

Tissue Specificity :

Detected on macrophages (at protein level). Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.

Background :

CD14, Cluster of differentiation 14, ingle-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. By in situ hybridization and study of somatic cell hybrid DNA that the gene is located at bands 5q23-q31. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.

Research Category :

Cell Type Markers, Hematopoietic Progenitors, Immunology, Innate Immunity, Mesenchymal Stem Cells, Myeloid, Stem Cells, Tlr Signaling