ELISA/assay

Human CCL18/PARC ELISA Kit PicoKine®

EK0686

1

Human CCL18/PARC ELISA Kit PicoKine®

human-ccl18-parc-picokine-trade-elisa-kit-ek0686-boster

Picokine ELISA Kits

CCL18

369

7.8 pg/ml - 500 pg/ml

Human CCL18/PARC ELISA Kit PicoKine (96 Tests). Quantitate Human CCL18 in cell culture supernatants and serum. Sensitivity: 10pg/ml.

Human CCL18/PARC ELISA Kit PicoKine (96 Tests). Quantitate Human CCL18 in cell culture supernatants and serum. Sensitivity: 10pg/ml.

96 wells/kit, with removable strips.

P55774

Expression system for standard: E.coli; Immunogen sequence: A21-A89

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

10 ng/tube

cell culture supernatants and serum.

Dilution ratio of 1:500, concentration in serum is around 40ng/ml

ELISA

Human

/e/k/ek0686.png

Human CCL18/PARC PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.017 0.071 0.102 0.177 0.348 0.680 1.252 1.964

5/15/14 22:17

3/2/22 2:54

Human CCL18/PARC ELISA Kit Price PicoKine® BosterBio

Human CCL18/PARC ELISA Kit, AMAC 1 ELISA Kit, Bosterbio High Sensitivity

Human CCL18/PARC ELISA Kit PicoKine (96 Tests). Quantitate Human CCL18 in cell culture supernatants and serum. Sensitivity: 10pg/ml.

99.1

500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.3 pg/ml, 15.6 pg/ml, 7.8 pg/ml

Chemokine (C-C motif) ligand 18 (CCL18) is a small cytokine belonging to the CC chemokine family that was previously called PARC (pulmonary and activation-regulated chemokine). CCL18 is approximately 60% identical in amino acid sequence to CCL3. By analysis of a previously mapped CCL18 from 17q11.2, it is determined that the PARC gene is located within 1 of the 2 clusters of CC chemokine genes in this region. It is expressed at high levels in lung and at lower levels in certain lymphoid tissues, such as the lymph nodes, and is chemotactic for activated T cells and nonactivated lymphocytes. Beside, CCL18 recruits Th2 cells and basophils and may play a predominant role in allergic asthma.

Cancer,Chemokines,Immunology,Innate Immunity,Oncoproteins,Tumor Biomarkers

C-C motif chemokine 18;Alternative macrophage activation-associated CC chemokine 1;AMAC-1;CC chemokine PARC;Dendritic cell chemokine 1;DC-CK1;Macrophage inflammatory protein 4;MIP-4;Pulmonary and activation-regulated chemokine;Small-inducible cytokine A18;CCL18 (1-68);CCL18 (3-69);CCL18 (4-69);CCL18;AMAC1, DCCK1, MIP4, PARC, SCYA18;

C-C motif chemokine 18

9849 MW

Chemotactic factor that attracts lymphocytes but not monocytes or granulocytes. May be involved in B-cell migration into B-cell follicles in lymph nodes. Attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses.

Secreted.

Expressed at high levels in lung, lymph nodes, placenta, bone marrow, dendritic cells present in germinal centers and T-cell areas of secondary lymphoid organs and macrophages derived from peripheral blood monocytes. Not expressed by peripheral blood monocytes and a monocyte-to-macrophage differentiation is a prerequisite for expression. Expressed in synovial fluids from patients with rheumatoid and septic arthritis and in ovarian carcinoma ascitic fluid.

C-C motif chemokine 18

Belongs to the intercrine beta (chemokine CC) family.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 18 49 269 18 52 279 Standard deviation 0.99 3.52 20.17 1.29 4.62 22.59 CV (%) 5.5% 7.2% 7.5% 7.2% 8.9% 8.1%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 18 17 18 18 17 0.43 2.5% Sample 2 49 56 49 48 50 3.2 6.4% Sample 3 269 295 278 296 284 11.45 4% *number of samples for each test n=16.

15-25min

monoclonal antibody from mouse polyclonal antibody from goat

23831213, 23697837

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