ELISA/assay

Human CA9/Carbonic anhydrase 9 ELISA Kit PicoKine®

EK0682

1

Human CA9/Carbonic anhydrase 9 ELISA Kit PicoKine®

human-ca9-picokine-trade-elisa-kit-ek0682-boster

Picokine ELISA Kits

CA9

369

15.6 pg/ml - 1,000 pg/ml

Human CA9/Ca Ix ELISA Kit PicoKine (96 Tests). Quantitate Human CA9 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 10pg/ml.

Human CA9/Ca Ix ELISA Kit PicoKine (96 Tests). Quantitate Human CA9 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 10pg/ml.

96 wells/kit, with removable strips.

Q16790

Expression system for standard: NS0; Immunogen sequence: P59-D414

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

10 ng/tube

cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

ELISA

Human

/antibody/ek0682-2-ELISA-human-ca9-ca-ix-picokine-elisa-kit.jpg

Human CA9 PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.03 0.086 0.128 0.231 0.395 0.77 1.506 2.265

5/15/14 22:17

3/2/22 2:54

Human CA9/Carbonic anhydrase 9 ELISA Kit Price PicoKine®

Human CA9 ELISA Kit, ca ix ELISA Kit, Bosterbio High Sensitivity

Human CA9/Ca Ix ELISA Kit PicoKine (96 Tests). Quantitate Human CA9 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 10pg/ml.

97

heparin, EDTA or citrate

1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.2 pg/ml, 15.6 pg/ml

Carbonic anhydrase 9 (CA9/CAIX) is an enzyme that in humans is encoded by the CA9 gene. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene is mapped to 9p13.3. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation.

Adaptive Immunity,Immunology,T Cells

Carbonic anhydrase 9;4.2.1.1;Carbonate dehydratase IX;Carbonic anhydrase IX;CA-IX;CAIX;Membrane antigen MN;P54/58N;Renal cell carcinoma-associated antigen G250;RCC-associated antigen G250;pMW1;CA9;G250, MN;

Carbonic anhydrase 9

49698 MW

Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.

Nucleus . Nucleus, nucleolus . Cell membrane ; Single-pass type I membrane protein . Cell projection, microvillus membrane ; Single-pass type I membrane protein . Found on the surface microvilli and in the nucleus, particularly in nucleolus.

Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.

Carbonic anhydrase 9

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 37 196 412 33 176 428 Standard deviation 2.84 10.97 27%3 2.77 11.61 29.96 CV (%) 7.7% 5.6% 6.1% 8.4% 6.6% 7%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 37 32 32 33 33 2.06 6.2% Sample 2 196 230 215 208 212 12.29 5.7% Sample 3 412 423 456 402 423 20.31 4.8% *number of samples for each test n=16.

15-25min

monoclonal antibody from mouse polyclonal antibody from goat

23225450

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