ELISA/assay

Human Cystatin C/CST3 ELISA Kit PicoKine®

EK0678

1

Human Cystatin C/CST3 ELISA Kit PicoKine®

human-cystatin-c-picokine-trade-elisa-kit-ek0678-boster

Picokine ELISA Kits

CST3

400

312 pg/ml - 20,000 pg/ml

Human Cystatin C/CST3 ELISA Kit PicoKine (96 Tests). Quantitate Human CST3 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. Sensitivity: 10pg/ml.

Human Cystatin C/CST3 ELISA Kit PicoKine (96 Tests). Quantitate Human CST3 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. Sensitivity: 10pg/ml.

96 wells/kit, with removable strips.

P01034

Expression system for standard: NS0; Immunogen sequence: M1-A146

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

20 ng/tube

cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk.

Dilution ratio of 1:500, concentration in serum is around 1500ng/ml

ELISA

Human

/e/k/ek0678.png

Human Cystatin C PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.036 0.095 0.185 0.290 0.581 0.952 1.350 1.923

7/13/14 22:17

3/2/22 2:54

Human Cystatin C/CST3 ELISA Kit Price PicoKine® BosterBio

Human Cystatin C/CST3 ELISA Kit PicoKine, Bosterbio High Sensitivity

100

heparin or EDTA

20,000 pg/ml, 10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 312 pg/ml

Cystatin C or cystatin 3 (formerly gamma trace, post-gamma-globulin or neuroendocrine basic polypeptide), a protein encoded by the CST3 gene, was originally described as a constituent of normal cerebrospinal fluid (CSF) and of urine from patients with renal failure.1 Cystatin 3 has a low molecular weight (approximately 13.3 kilodaltons), and it is removed from the bloodstream by glomerular filtration in the kidneys. In humans, all cells with a nucleus (cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases2 (enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases3 (it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Moreover, cystatin C is involved in network reorganization in the epileptic dentate gyrus.4

Apoptosis,Atherosclerosis,Cardiovascular,Caspases Etc,Cell Biology,Cell Type Markers,Intracellular,Neural Stem Cells,Stem Cells,Tags & Cell Markers,Tumor Associated

Cystatin-C;Cystatin-3;Gamma-trace;Neuroendocrine basic polypeptide;Post-gamma-globulin;CST3;

Cystatin-C

15799 MW

As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.

Secreted .

Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland.

Cystatin-C

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 687 2837 10374 647 2715 10112 Standard deviation 43.96 207.1 684.68 51.11 217.2 859.52 CV (%) 6.4% 7.3% 6.6% 7.9% 8% 8.5%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 687 650 665 676 669 13.68 2% Sample 2 2837 2995 2880 2796 2877 74.32 2.5% Sample 3 10374 8987 8970 10046 9594 626.6 6.5% *number of samples for each test n=16.

15-25min

monoclonal antibody from mouse polyclonal antibody from goat

24963810, 25216761, 24821156, 24661756, 22120484

elisa-kits