ELISA/assay

Human Cathepsin D ELISA Kit PicoKine®

EK0672

1

Human Cathepsin D ELISA Kit PicoKine®

human-cathepsin-d-picokine-trade-elisa-kit-ek0672-boster

Picokine ELISA Kits

CTSD

430

156 pg/ml - 10,000 pg/ml

Human Cathepsin D ELISA Kit PicoKine (96 Tests). Quantitate Human CTSD in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Human Cathepsin D ELISA Kit PicoKine (96 Tests). Quantitate Human CTSD in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

96 wells/kit, with removable strips.

P07339

Expression system for standard: NS0; Immunogen sequence: L21-L412

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

10 ng/tube

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Dilution ratio of 1:100, concentration in serum is 125-250ng/ml

ELISA

Human

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Human Cathepsin D PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.004 0.064 0.118 0.207 0.409 0.661 1.243 1.861

5/15/14 22:17

3/2/22 2:54

Human Cathepsin D ELISA Kit Price PicoKine® BosterBio

Human Cathepsin D ELISA Kit, CATD HUMAN ELISA, Bosterbio High Sensitivity

Human Cathepsin D ELISA Kit PicoKine (96 Tests). Quantitate Human CTSD in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

96.5

heparin or EDTA

10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 312 pg/ml, 156 pg/ml

Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.

Cancer,Cell Adhesion Proteins,Cell Biology,Cytoskeleton / Ecm,Ecm Enzymes,Extracellular Matrix,Invasion/Microenvironment,Membrane Proteins,Neuroscience,Proteolysis / Ubiquitin,Proteolytic Enzymes,Signal Transduction

Cathepsin D;3.4.23.5;Cathepsin D light chain;Cathepsin D heavy chain;CTSD;CPSD;

Cathepsin D

44552 MW

Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.

Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).

Expressed in the aorta extrcellular space (at protein level).

Cathepsin D

Belongs to the peptidase A1 family.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 189 1630 5138 181 1712 5150 Standard deviation 13.6 91.28 339.1 13.75 111.28 427.45 CV (%) 7.2% 5.6% 6.6% 7.6% 6.5% 8.3%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 189 190 216 201 199 10.88 5.4% Sample 2 1630 1733 1553 1567 1620 71 4.3% Sample 3 5138 5161 4737 4988 5006 168.91 3.3% *number of samples for each test n=16.

15-25min

monoclonal antibody from mouse polyclonal antibody from goat

11331669

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