ELISA/assay

Mouse Cathepsin B ELISA Kit PicoKine®

EK0671

1

Mouse Cathepsin B ELISA Kit PicoKine®

mouse-cathepsin-b-picokine-trade-elisa-kit-ek0671-boster

Picokine ELISA Kits

CTSB

400

156 pg/ml - 10,000 pg/ml

Mouse Cathepsin B ELISA Kit PicoKine (96 Tests). Quantitate Mouse Ctsb in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.

Mouse Cathepsin B ELISA Kit PicoKine (96 Tests). Quantitate Mouse Ctsb in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.

96 wells/kit, with removable strips.

P10605

Expression system for standard: NS0; Immunogen sequence: H18-F339

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<5 pg/ml

10 ng/tube

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Dilution ratio of 1:5000, concentration in serum is 5-10ug/ml

ELISA

Mouse

/e/k/ek0671_2.png

Mouse Cathepsin B PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.014 0.102 0.170 0.293 0.532 0.800 1.301 1.929

5/16/14 12:17

3/2/22 2:54

Mouse Cathepsin B ELISA Kit Price PicoKine® BosterBio

Mouse Cathepsin B ELISA Kit, APPS ELISA Kit, Bosterbio High Sensitivity

Mouse Cathepsin B ELISA Kit PicoKine (96 Tests). Quantitate Mouse Ctsb in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.

100

heparin or EDTA

10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 312 pg/ml, 156 pg/ml

Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene.1, 2 And this gene is mapped to chromosome 8p22.3 The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease.4 Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. The standard product used in this kit is recombinant mouse Cathepsin B with the molecular mass of 37KDa.

Angiogenesis,Cancer,Cell Adhesion,Cell Adhesion Proteins,Cytoskeleton / Ecm,Ecm Enzymes,Extracellular Matrix,Invasion/Microenvironment,Membrane Proteins,Neuroscience,Signal Transduction

Cathepsin B;3.4.22.1;Cathepsin B1;Cathepsin B light chain;Cathepsin B heavy chain;Ctsb;

Cathepsin B

37280 MW

Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.

Lysosome. Melanosome . Secreted, extracellular space .

Cathepsin B

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 242 1828 4749 229 1916 4534 Standard deviation 14.76 100.54 370.42 13.96 128.37 439.79 CV (%) 6.1% 5.5% 7.8% 6.1% 6.7% 9.7%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 242 214 229 235 230 10.31 4.4% Sample 2 1828 1771 1721 1761 1770 38.23 2.1% Sample 3 4749 4980 5227 4633 4897 227.69 4.6% *number of samples for each test n=16.

15-25min

monoclonal antibody from rat polyclonal antibody from goat

23828190

elisa-kits