Human Cathepsin B ELISA Kit PicoKine¬Æ | Boster EK0670 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human Cathepsin B ELISA Kit PicoKine¬Æ

catalog :

EK0670

more info or order :

Boster product webpage

citations: 1

Reference
Chen Q, Fei J, Wu L, Jiang Z, Wu Y, Zheng Y, et al. Detection of cathepsin B, cathepsin L, cystatin C, urokinase plasminogen activator and urokinase plasminogen activator receptor in the sera of lung cancer patients. Oncol Lett. 2011;2:693-699 pubmed

image

image 1 :

Human Cathepsin B PicoKine ELISA Kit standard curve

product information

sku :

EK0670

status :

name :

Human Cathepsin B ELISA Kit PicoKine¬Æ

url key :

human-cathepsin-b-picokine-trade-elisa-kit-ek0670-boster

product category :

Picokine ELISA Kits

gene name :

CTSB

price :

400

assay range :

156 pg/ml - 10,000 pg/ml

description :

Human Cathepsin B ELISA Kit PicoKine (96 Tests). Quantitate Human CTSB in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.

short description :

Human Cathepsin B ELISA Kit PicoKine (96 Tests). Quantitate Human CTSB in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

P07858

immunogen :

Expression system for standard: NS0; Immunogen sequence: R18-I339

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<5 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

application details :

Dilution ratio of 1:40, concentration in serum is around 100ng/ml

applications :

ELISA

reactivity :

Human

images :

/e/k/ek0670_1.png

image labels :

Human Cathepsin B PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.048 0.217 0.299 0.522 0.897 1.074 1.548 1.923

created at :

5/16/14 12:17

updated at :

3/2/22 2:54

meta title :

Human Cathepsin B ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Human Cathepsin B ELISA Kit, APPS ELISA Kit, Bosterbio High Sensitivity

meta description :

Human Cathepsin B ELISA Kit PicoKine (96 Tests). Quantitate Human CTSB in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.

rating value :

97.6

anticoagulant :

heparin or EDTA

assay procedure step 1 :

10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 312 pg/ml, 156 pg/ml

background :

Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene.1, 2 And this gene is mapped to chromosome 8p22.3 The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease.4 Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. The standard product used in this kit is recombinant human Cathepsin B with the molecular mass of 37KDa.

research category :

Angiogenesis,Cancer,Cell Adhesion,Cell Adhesion Proteins,Cytoskeleton / Ecm,Ecm Enzymes,Extracellular Matrix,Invasion/Microenvironment,Membrane Proteins,Neuroscience,Signal Transduction

synonyms :

Cathepsin B;3.4.22.1;APP secretase;APPS;Cathepsin B1;Cathepsin B light chain;Cathepsin B heavy chain;CTSB;CPSB;

gene full name :

Cathepsin B

molecular weight :

37822 MW

protein function :

Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.

subcellular localization :

Lysosome. Melanosome. Secreted, extracellular space . Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

protein name :

Cathepsin B

sequence similarities :

Belongs to the peptidase C1 family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 252 1856 4296 234 1923 4064 Standard deviation 17.64 129.92 326.49 17.85 148.07 377.95 CV (%) 7% 7% 7.6% 7.8% 7.7% 9.3%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 252 275 235 272 258 16.19 6.2% Sample 2 1856 1776 1691 1826 1787 62.48 3.4% Sample 3 4296 4210 3832 4617 4238 279.56 6.5% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

polyclonal antibody from goat polyclonal antibody from goat

normal range citations :

22848251

template :

elisa-kits

more info or order :

Boster product webpage