ELISA/assay

Human N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine®

EK0669

1

Human N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine®

human-cadherin-2-n-cadherin-picokine-trade-elisa-kit-ek0669-boster

Picokine ELISA Kits

CDH2

369

156 pg/ml - 10,000 pg/ml

Human N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine (96 Tests). Quantitate Human CDH2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml.

Human N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine (96 Tests). Quantitate Human CDH2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml.

96 wells/kit, with removable strips.

P19022

Expression system for standard: NS0; Immunogen sequence: D160-A724

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<20 pg/ml

50 ng/tube

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Dilution ratio of 1:1, concentration in serum is around 1500pg/ml

ELISA

Human

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Human Cadherin-2/N-Cadherin PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.019 0.121 0.214 0.384 0.732 1.020 1.810 2.121

7/6/14 15:18

3/2/22 2:54

Human N-Cadherin-2 CDH2 CD325 ELISA Kit Price PicoKine®

Human Cadherin-2/N-Cadherin ELISA Kit, Cadherin 2 ELISA

Human N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine (96 Tests). Quantitate Human CDH2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml.

97.2

heparin or EDTA

10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 312 pg/ml, 156 pg/ml

Cadherin-2 (CDH2), also known as neural cadherin (NCAD), is a protein that in humans is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. Cadherin-2 is expressed in the brain, skeletal and cardiac muscle. Cadherin-2 is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.

Cadherins,Cell Adhesion,Cytoskeleton / Ecm,Signal Transduction

Cadherin-2;CDw325;Neural cadherin;N-cadherin;CD325;CDH2;CDHN, NCAD;

Cadherin-2

99809 MW

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density (By similarity).

Cell membrane; Single-pass type I membrane protein.

Cadherin-2

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 223 1172 4928 308 1190 4953 Standard deviation 13.16 50.40 202.05 20.33 69.02 376.43 CV (%) 5.9% 4.3% 4.1% 6.6% 5.8% 7.6%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 223 193 211 223 212 12.27 5.7% Sample 2 1172 1262 1146 1232 1203 46.18 3.8% Sample 3 4928 5617 4816 5488 5212 345.56 6.6% *number of samples for each test n=16.

15-25min

monoclonal antibody from mouse polyclonal antibody from goat

23799912

elisa-kits