Mouse BAFF ELISA Kit PicoKine¬Æ | Boster EK0664 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse BAFF ELISA Kit PicoKine¬Æ

catalog :

EK0664

more info or order :

Boster product webpage

citations: 1

Reference
Kim J, Seo G, Kim P. Activin A Stimulates Mouse APCs to Express BAFF via ALK4-Smad3 Pathway. Immune Netw. 2011;11:196-202 pubmed publisher

image

image 1 :

Mouse BAFF PicoKine ELISA Kit standard curve

product information

sku :

EK0664

status :

name :

Mouse BAFF ELISA Kit PicoKine¬Æ

url key :

mouse-baff-picokine-trade-elisa-kit-ek0664-boster

product category :

Picokine ELISA Kits

gene name :

TNFSF13B

price :

400

assay range :

46.9 pg/ml - 3,000 pg/ml

description :

Mouse BAFF ELISA Kit PicoKine (96 Tests). Quantitate Mouse Tnfsf13b in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

short description :

Mouse BAFF ELISA Kit PicoKine (96 Tests). Quantitate Mouse Tnfsf13b in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

Q3KP92

immunogen :

Expression system for standard: NS0; Immunogen sequence: A127-L309

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<10 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

application details :

Dilution ratio of 1:50, concentration in serum is 20-30ng/ml

applications :

ELISA

reactivity :

Mouse

images :

/e/k/ek0664_2.png

image labels :

Mouse BAFF PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 46.9 94 188 375 750 1500 3000 O.D. 0.037 0.107 0.157 0.247 0.454 0.764 1.214 1.931

created at :

5/17/14 2:17

updated at :

3/2/22 2:54

meta title :

Mouse BAFF ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Mouse BAFF ELISA Kit, ApoL related ligand TALL 1 ELISA

meta description :

Mouse BAFF ELISA Kit PicoKine (96 Tests). Quantitate Mouse Tnfsf13b in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

rating value :

100

anticoagulant :

heparin or EDTA

assay procedure step 1 :

3,000 pg/ml, 1,500 pg/ml, 750 pg/ml, 375 pg/ml, 188 pg/ml, 94 pg/ml, 46.9 pg/ml

background :

B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFLSF13B gene.1,2 BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells.3All these ligands act as heterotrimers (i.e. three of the same molecule) interacting with heterotrimeric receptors4, although BAFF has been known to be active as either a hetero- or homotrimer.5 The standard used in this kit is recombinant mouse soluble BAFF (A127-L309) with molecular weight 23.2KDa.

research category :

Adaptive Immunity,B Cells,Cancer,Cytokines,Growth Factors,Growth Factors/Hormones,Immunology,Innate Immunity,Non-Cd,Signal Transduction,Tnf Superfamily

synonyms :

Tumor necrosis factor (Ligand) superfamily, member 13b ;Tumor necrosis factor (Ligand) superfamily, member 13b, isoform CRA_d ;Tnfsf13b ;mCG_3400 ;

gene full name :

tumor necrosis factor (ligand) superfamily, member 13b

molecular weight :

34192 MW

protein name :

Tumor necrosis factor ligand superfamily member 13B

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 82 287 1221 76 287 1209 Standard deviation 6.47 15.52 67.15 7.14 16.07 72.54 CV (%) 7.9% 5.3% 5.5% 9.4% 5.6% 6%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 82 85 90 80 84 3.76 4.4% Sample 2 293 335 318 295 310 17.34 5.5% Sample 3 1221 1161 1374 1312 1267 81.89 6.4% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

monoclonal antibody from rat polyclonal antibody from goat

normal range citations :

22039367

template :

elisa-kits

more info or order :

Boster product webpage