Human BAFF / TNFSF13B ELISA Kit PicoKine¬Æ | Boster EK0663 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human BAFF / TNFSF13B ELISA Kit PicoKine¬Æ

catalog :

EK0663

more info or order :

Boster product webpage

citations: 2

Reference
El Kissi Y, Samoud S, Mtiraoui A, Letaief L, Hannachi N, Ayachi M, et al. Increased Interleukin-17 and decreased BAFF serum levels in drug-free acute schizophrenia. Psychiatry Res. 2015;225:58-63 pubmed publisher
Bornacelly A, Mercado D, Acevedo N, Caraballo L. The strength of the antibody response to the nematode Ascaris lumbricoides inversely correlates with levels of B-Cell Activating Factor (BAFF). BMC Immunol. 2014;15:22 pubmed publisher

image

image 1 :

Human BAFF PicoKine ELISA Kit standard curve

product information

sku :

EK0663

status :

name :

Human BAFF / TNFSF13B ELISA Kit PicoKine¬Æ

url key :

human-baff-picokine-trade-elisa-kit-ek0663-boster

product category :

Picokine ELISA Kits

gene name :

TNFSF13B

price :

400

assay range :

62.5 pg/ml - 4,000 pg/ml

description :

Human BAFF / TNFSF13B ELISA Kit PicoKine (96 Tests). Quantitate Human TNFSF13B in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml.

short description :

Human BAFF / TNFSF13B ELISA Kit PicoKine (96 Tests). Quantitate Human TNFSF13B in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

Q9Y275

immunogen :

Expression system for standard: NS0; Immunogen sequence: A134-L285

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<2 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

application details :

Dilution ratio of 1:4, concentration in serum is around 4ng/ml

applications :

ELISA

reactivity :

Human

images :

/e/k/ek0663.png

image labels :

Human BAFF PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.038 0.072 0.121 0.179 0.311 0.548 0.935 1.829

created at :

5/17/14 2:17

updated at :

3/2/22 2:54

meta title :

Human BAFF / TNFSF13B ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Human BAFF ELISA Kit, ApoL related ligand TALL 1 ELISA

meta description :

Human BAFF / TNFSF13B ELISA Kit PicoKine (96 Tests). Quantitate Human TNFSF13B in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml.

rating value :

97.1

anticoagulant :

heparin or EDTA

assay procedure step 1 :

4,000 pg/ml, 2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml

background :

BAFF was regularly detected by enzyme-linked immunosorbent assay in brain tissue lysates and in normal spinal fluid, and in astrocytes by double fluorescence microscopy. BAFF was localized in astrocytes close to BAFF-R-expressing immune cells. BAFF receptors were strongly expressed in situ in primary central nervous system (CNS) lymphomas.1 The TNF superfamily member B cell-activating factor (BAFF) plays an important role in humoral immunity and in autoimmune diseases, including RA.Local BAFF gene targeting inhibited proinflammatory cytokine expression, suppressed generation of plasma cells and Th17 cells, and markedly ameliorated joint pathology.2 The B cell activating factor BAFF (BlyS/TALL-1/zTNF4) is a tumor necrosis factor (TNF)-related ligand that promotes B cell survival and binds to three receptors (BCMA, TACI, and the recently described BAFF-R).3 Human BAFF was mapped to chromosome 13q32-34.4 The standard used in this kit is recombinant soluble human BAFF (A134-L295) with the molecular mass of 19.6KDa.

research category :

Adaptive Immunity,B Cells,Cancer,Cytokines,Growth Factors,Growth Factors/Hormones,Immunology,Innate Immunity,Non-Cd,Signal Transduction,Tnf Superfamily

synonyms :

Tumor necrosis factor ligand superfamily member 13B;B lymphocyte stimulator;BLyS;B-cell-activating factor;BAFF;Dendritic cell-derived TNF-like molecule;TNF- and APOL-related leukocyte expressed ligand 1;TALL-1;CD257;Tumor necrosis factor ligand superfamily member 13b, membrane form;Tumor necrosis factor ligand superfamily member 13b, soluble form;TNFSF13B;BAFF, BLYS, TALL1, TNFSF20, ZTNF4;UNQ401/PRO738;

gene full name :

Tumor necrosis factor ligand superfamily member 13B

molecular weight :

31223 MW

protein function :

Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.

subcellular localization :

Cell membrane; Single-pass type II membrane protein.

tissue specificity :

Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T- cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas. Isoform 2 is expressed in many myeloid cell lines.

protein name :

Tumor necrosis factor ligand superfamily member 13B

sequence similarities :

Belongs to the tumor necrosis factor family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 127 522 1229 124 548 1268 Standard deviation 5.46 38.62 79.88 5.7 47.67 103.97 CV (%) 4.3% 7.4% 6.5% 4.6% 8.7% 8.2%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 127 125 149 127 132 9.84 7.4% Sample 2 522 513 536 528 524 8.4 1.6% Sample 3 1229 1085 1215 1223 1188 59.67 5% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

normal range citations :

25453636, 24906685

template :

elisa-kits

more info or order :

Boster product webpage