ELISA/assay

Human AXL ELISA Kit PicoKine®

EK0659

1

Human AXL ELISA Kit PicoKine®

human-axl-picokine-trade-elisa-kit-ek0659-boster

Picokine ELISA Kits

AXL

369

62.5 pg/ml - 4,000 pg/ml

Human AXL ELISA Kit PicoKine (96 Tests). Quantitate Human AXL in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Human AXL ELISA Kit PicoKine (96 Tests). Quantitate Human AXL in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

96 wells/kit, with removable strips.

P30530

Expression system for standard: NS0; Immunogen sequence: E33-P440

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

10 ng/tube

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

ELISA

Human

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Human AXL PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.063 0.09 0.135 0.199 0.345 0.655 1.155 2.066

5/17/14 9:17

3/2/22 2:54

Human AXL ELISA Kit Price PicoKine® BosterBio

Human AXL ELISA Kit, AXL oncogene ELISA Kit, Bosterbio High Sensitivity

Human AXL ELISA Kit PicoKine (96 Tests). Quantitate Human AXL in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

98.7

heparin or EDTA

4,000 pg/ml, 2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml

Tyrosine-protein kinase receptor UFO is an enzyme that in humans is encoded by the AXL gene. The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, the Axl protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. It is involved in the stimulation of cell proliferation. This receptor can also mediate cell aggregation by homophilic binding. Axl is a chronic myelogenous leukemia-associated oncogene and also associated with colon cancer and melanoma. It is in close vicinity to the bcl3 oncogene, which is at 19q13.1-q13.2. The Axl gene is evolutionarily conserved between vertebrate species. This gene has two different alternatively spliced transcript variants.

sc 1097 sc 20741 sc 1096 sc 166269 sc 166268

Cancer,Cell Biology,Cell Cycle,Cell Type Markers,Kinases/Phosphatases,Oncoproteins,Oncoproteins/Suppressors,Protein Phosphorylation,Receptor Tyrosine Kinases,Signal Transduction,Tags & Cell Markers,Tumor Associated,Tyrosine Kinases

Tyrosine-protein kinase receptor UFO;2.7.10.1;AXL oncogene;AXL;UFO;

Tyrosine-protein kinase receptor UFO

98336 MW

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, ALX binds and induces tyrosine phosphorylation of PI3- kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response. In case of filovirus infection, seems to function as a cell entry factor.

Cell membrane ; Single-pass type I membrane protein .

Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.

Tyrosine-protein kinase receptor UFO

Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 103 593 12107 107 550 1194 Standard deviation 8.13 45.06 86.76 9.3 49.5 86.7%6 CV (%) 7.9% 7.6% 6.7% 8.7% 9% 7.2%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 103 88 85 85 90 7.46 8.2% Sample 2 593 706 654 601 638 45.47 7.1% Sample 3 1295 1325 1154 1246 1255 64.77 5.1% *number of samples for each test n=16.

15-25min

monoclonal antibody from mouse polyclonal antibody from goat

24239956

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