ELISA/assay

Human Angiopoietin-2 / ANG2 ELISA Kit PicoKine®

EK0657

1

Human Angiopoietin-2 / ANG2 ELISA Kit PicoKine®

human-angiopoietin-2-picokine-trade-elisa-kit-ek0657-boster

Picokine ELISA Kits

ANGPT2

369

156 pg/ml - 10,000 pg/ml

Human Angiopoietin-2 / ANG2 ELISA Kit PicoKine (96 Tests). Quantitate Human ANGPT2 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and human milk. Sensitivity: 10pg/ml.

Human Angiopoietin-2 / ANG2 ELISA Kit PicoKine (96 Tests). Quantitate Human ANGPT2 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and human milk. Sensitivity: 10pg/ml.

96 wells/kit, with removable strips.

O15123

Expression system for standard: NS0; Immunogen sequence: Y19-F496

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

10 ng/tube

cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and human milk.

Dilution ratio of 1:1, concentration in serum is around 2ng/ml

ELISA

Human

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Human Angiopoietin-2 PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.095 0.178 0.210 0.376 0.519 0.970 1.588 2.010

5/17/14 9:17

3/2/22 2:54

Human Angiopoietin-2 / ANG2 ELISA Kit Price PicoKine®

Human Angiopoietin-2 ELISA Kit PicoKine, Bosterbio High Sensitivity

Human Angiopoietin-2 / ANG2 ELISA Kit PicoKine (96 Tests). Quantitate Human ANGPT2 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and human milk. Sensitivity: 10pg/ml.

100

heparin or EDTA

10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 312 pg/ml, 156 pg/ml

Angiopoietin-2 is a protein that in humans is encoded by the ANGPT2 gene. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. ANGPT2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis. Its expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.

Angiogenesis,Cardiovascular,Growth Factors

Angiopoietin-2;ANG-2;ANGPT2;

Angiopoietin-2

56919 MW

Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.

Secreted.

Angiopoietin-2

Contains 1 fibrinogen C-terminal domain.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 327 1634 5900 341 1769 5438 Standard deviation 17.33 114.38 354 23.52 127.36 342.59 CV (%) 5.3% 7% 6% 6.9% 7.2% 6.3%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 327 351 360 317 338 17.41 5.1% Sample 2 1634 1902 1611 1928 1768 146.76 8.3% Sample 3 5900 6201 5314 5672 5771 324.1 5.6% *number of samples for each test n=16.

15-25min

monoclonal antibody from mouse polyclonal antibody from goat

23823085, 23056802

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