Human Endoglin/CD105/ENG ELISA Kit PicoKine¬Æ | Boster EK0644 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human Endoglin/CD105/ENG ELISA Kit PicoKine¬Æ

catalog :

EK0644

more info or order :

Boster product webpage

citations: 1

Reference
Kopczynska E, Dancewicz M, Kowalewski J, Makarewicz R, Kardymowicz H, Kaczmarczyk A, et al. Influence of surgical resection on plasma endoglin (CD105) level in non‑small cell lung cancer patients. Exp Oncol. 2012;34:53-6 pubmed

image

image 1 :

Human CD105 PicoKine ELISA Kit standard curve

product information

sku :

EK0644

status :

name :

Human Endoglin/CD105/ENG ELISA Kit PicoKine¬Æ

url key :

human-cd105-picokine-trade-elisa-kit-ek0644-boster

product category :

Picokine ELISA Kits

gene name :

ENG

price :

400

assay range :

156 pg/ml - 10,000 pg/ml

description :

Human CD105 ELISA Kit PicoKine (96 Tests). Quantitate Human ENG in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml.

short description :

Human CD105 ELISA Kit PicoKine (96 Tests). Quantitate Human ENG in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

P17813

immunogen :

Expression system for standard: CHO; Immunogen sequence: E26-G586

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<15 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

applications :

ELISA

reactivity :

Human

images :

/e/k/ek0644.png

image labels :

Human CD105 PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.011 0.122 0.258 0.471 0.766 1.312 1.658 2.022

created at :

5/17/14 9:17

updated at :

3/2/22 2:54

description before attributes :

Featured Product . Fast ELISA kit now available: Boster is proud to announce the newest Picokine Fast ELISA kits. Now you can perform what used to be 4+ hour ELISA assay in under 1.5 hours. Available for Human CD105 PicoKine Fast ELISA Kit and many others.

meta title :

Human Endoglin/CD105/ENG ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Human CD105 ELISA Kit, Endoglin ELISA Kit, Bosterbio High Sensitivity

rating value :

97.9

anticoagulant :

heparin or EDTA

assay procedure step 1 :

10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 312 pg/ml, 156 pg/ml

background :

CD105, also called Endoglin, is a homodimeric membrane glycoprotein primarily associated with human vascular endothelium. It is also found on bone marrow proerythroblasts, activated monocytes, and lymphoblasts in childhood leukemia. Endoglin is a component of the transforming growth factor-beta (TGFB) receptor complex and binds TGFB1 with high affinity.1 CD105 gene is mapped to 9q34.1. The coding region of the gene contains 14 exons.2 The protein consists of a homodimer of 180 kDA with disulfide links. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. Furthermore, it also has a role in the balance of ALK1 and ALK5 signaling to regulate endothelial cell proliferation.3 Moreover, the elevated expression of endoglin in the surgically excised CNVMs suggested a persisting postmitotic activation in an advanced stage of neovascular tissue.4 The standard product used in this kit is extracellular part of recombinant human CD105, from E26 to G586. As a result of glycosylation, the molecular mass is 75-85KDa.

research category :

Epigenetics And Nuclear Signaling,Neurogenesis,Neurology Process,Neuroscience,Transcription

synonyms :

Endoglin;CD105;ENG;END;

gene full name :

Endoglin

molecular weight :

70578 MW

protein function :

Major glycoprotein of vascular endothelium. Involved in the regulation of angiogenesis. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. Acts as TGF-beta coreceptor and is involved in the TGF- beta/BMP signaling cascade. Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGF-beta1 signaling through SMAD3.

subcellular localization :

Membrane; Single-pass type I membrane protein.

tissue specificity :

Endoglin is restricted to endothelial cells in all tissues except bone marrow.

protein name :

Endoglin

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 312 1384 4821 316 1275 4955 Standard deviation 16.22 74.73 318.18 17.38 72.67 386.49 CV (%) 5.2% 5.4% 6.6% 5.5% 5.7% 7.8%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 312 285 280 306 295 13.53 4.5% Sample 2 1384 1301 1412 1238 1333 68.71 5.1% Sample 3 4821 5271 4779 5474 5086 295.48 5.8% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

normal range citations :

22453150

template :

elisa-kits

more info or order :

Boster product webpage