ELISA/assay

Human Survivin ELISA Kit PicoKine®

EK0641

1

Human Survivin ELISA Kit PicoKine®

human-survivin-picokine-trade-elisa-kit-ek0641-boster

Picokine ELISA Kits

BIRC5

430

62.5 pg/ml - 4,000 pg/ml

Human Survivin ELISA Kit PicoKine (96 Tests). Quantitate Human BIRC5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml.

Human Survivin ELISA Kit PicoKine (96 Tests). Quantitate Human BIRC5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml.

96 wells/kit, with removable strips.

O15392

Expression system for standard: E.coli; Immunogen sequence: M1-D142

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<2 pg/ml

4 ng/tube

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit.

ELISA

Human

/e/k/ek0641_1.png

Human Survivin PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.050 0.122 0.178 0.245 0.431 0.733 1.275 1.902

5/17/14 9:17

3/2/22 2:54

Human Survivin ELISA Kit Price PicoKine® BosterBio

Human Survivin ELISA Kit, API4 ELISA Kit, Bosterbio High Sensitivity

Human Survivin ELISA Kit PicoKine (96 Tests). Quantitate Human BIRC5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml.

100

heparin or EDTA

4,000 pg/ml, 2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml

Survivin (also termed API4) belongs to the family of genes known as inhibitors of apoptosis, and it has been implicated in both prevention of cell death and control of mitosis. Survivin is prominently expressed in transformed cell lines and in all the most common human cancers of lung, colon, pancreas, prostate and breast in vivo. It is also found in approximately 50% of high-grade non-Hodgkin's lymphomas. Survivin encodes a deduced 142-amino acid protein and localizes on chromosome 17q25. The overexpression of it in cancer may overcome an apoptotic checkpoint and favour aberrant progression of transformed cells through mitosis. The standard product used in this kit is recombinant human Survivin with the molecular mass of 34 KDa.

Epigenetics And Nuclear Signaling,Transcription,Apoptosis,Cancer,Cell Biology,Intracellular,Transcription Factors,Cell Cycle,Cell Death,Nuclear,Cell Division,Chromosome Structure,Invasion/Microenvironment

Baculoviral IAP repeat-containing protein 5;Apoptosis inhibitor 4;Apoptosis inhibitor survivin;BIRC5;API4, IAP4;

Baculoviral IAP repeat-containing protein 5

16389 MW

Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.

Cytoplasm. Nucleus. Chromosome. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Midbody. Localizes on chromosome arms and inner centromeres from prophase through metaphase. Localizes to kinetochores in metaphase, distributes to the midzone microtubules in anaphase and at telophase, localizes exclusively to the midbody. Colocalizes with AURKB at mitotic chromosomes. Acetylation at Lys-129 directs its localization to the nucleus by enhancing homodimerization and thereby inhibiting XPO1/CRM1- mediated nuclear export.

Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.

Baculoviral IAP repeat-containing protein 5

Belongs to the IAP family.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 207 660 1259 192 690 1195 Standard deviation 15.52 48.18 51.61 14.13 57.27 66.92 CV (%) 7.5% 7.3% 4.1% 8.3% 8.3% 5.6%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 207 178 185 184 188 11.01 5.8% Sample 2 660 723 712 743 709 30.66 4.3% Sample 3 1259 1187 1079 1117 1160 68.8 5.9% *number of samples for each test n=16.

15-25min

monoclonal antibody from mouse polyclonal antibody from goat

25248821

elisa-kits