Rat MMP-2 ELISA Kit PicoKine¬Æ | Boster EK0639 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Rat MMP-2 ELISA Kit PicoKine¬Æ

catalog :

EK0639

more info or order :

Boster product webpage

citations: 1

Reference
Wang J, Zhang Q, Mei X, Zhang X. Hydroxysafflor yellow A attenuates left ventricular remodeling after pressure overload-induced cardiac hypertrophy in rats. Pharm Biol. 2014;52:31-5 pubmed publisher

image

image 1 :

Rat MMP-2 PicoKine ELISA Kit standard curve

product information

sku :

EK0639

status :

name :

Rat MMP-2 ELISA Kit PicoKine¬Æ

url key :

rat-mmp-2-picokine-trade-elisa-kit-ek0639-boster

product category :

Picokine ELISA Kits

gene name :

MMP2

price :

400

assay range :

625 pg/ml - 40,000 pg/ml

description :

Rat MMP-2 ELISA Kit PicoKine (96 Tests). Quantitate Rat Mmp2 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.

short description :

Rat MMP-2 ELISA Kit PicoKine (96 Tests). Quantitate Rat Mmp2 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

P33436

immunogen :

Expression system for standard: NS0; Immunogen sequence: A30-C662

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<10 pg/ml

kit components :

40 ng/tube

sample type :

cell culture supernatants, serum and plasma (heparin).

applications :

ELISA

reactivity :

Rat

images :

/e/k/ek0639_1.png

image labels :

Rat MMP-2 PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 625 1250 2500 5000 10000 20000 40000 O.D. 0.131 0.168 0.213 0.332 0.585 0.925 1.498 2.082

created at :

7/15/14 9:17

updated at :

3/2/22 2:54

meta title :

Rat MMP-2 ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Rat MMP-2 ELISA Kit, MMP2/MONA ELISA, Bosterbio High Sensitivity

meta description :

Rat MMP-2 ELISA Kit PicoKine (96 Tests). Quantitate Rat Mmp2 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.

rating value :

98.9

anticoagulant :

heparin

assay procedure step 1 :

40,000 pg/ml, 20,000 pg/ml, 10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml

background :

Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2 (MMP2). It is also known as gelatinase, 72-kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown.1 Matrix metalloproteinases (MMPs) catalyze extracellular matrix degradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue turnover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide (cysteine switch). The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability.2 The gene is localized to 16q21 using somatic cell hybrids and in situ hybridization.3 The standard product used in this kit is recombinant rat MMP-2, consisting of 662 amino acids with the molecular mass of 72KDa. The detected MMP-2 includes zymogen and active enzyme.

research category :

Angiogenesis,Cancer,Cancer Metabolism,Cardiovascular,Cell Biology,Extracellular Matrix,Invasion/Microenvironment,Metabolism,Metabolism Processes,Metalloprotease,Pathways And Processes,Proteolysis / Ubiquitin,Proteolytic Enzymes,Response To Hypoxia,Tumor Biomarkers

synonyms :

72 kDa type IV collagenase;3.4.24.24;72 kDa gelatinase;Gelatinase A;Matrix metalloproteinase-2;MMP-2;PEX;Mmp2;

gene full name :

72 kDa type IV collagenase

molecular weight :

74149 MW

protein function :

Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly- -Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues (By similarity).

subcellular localization :

Secreted, extracellular space, extracellular matrix . Membrane . Nucleus . Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes (By similarity).

protein name :

72 kDa type IV collagenase

sequence similarities :

Belongs to the peptidase M10A family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1093 4492 23073 1098 4440 21240 Standard deviation 68.85 296.47 1107.5 90.03 368.52 1296.64 CV (%) 8.3% 6.6% 4.8% 8.2% 8.3% 6.1%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1093 1090 975 1117 1068 55.12 5.1% Sample 2 4492 3849 4402 4495 4309 268.48 6.2% Sample 3 23073 23113 22919 26456 23890 1483.1 6.2% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

normal range citations :

24033225

template :

elisa-kits

more info or order :

Boster product webpage