Rat IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine¬Æ

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Rat IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine¬Æ

catalog :

EK0592

more info or order :

Boster product webpage

citations: 1

Reference
Zhou Z, Weng Q, Zhou J, Zhou J. Extracts of Salvia miltiorrhiza Bunge on the cytokines of rat endometriosis models. Afr J Tradit Complement Altern Med. 2012;9:303-14 pubmed

image

image 1 :

Rat IL-18 PicoKine ELISA Kit standard curve

product information

sku :

EK0592

status :

name :

Rat IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine¬Æ

url key :

rat-il-18-picokine-trade-elisa-kit-ek0592-boster

product category :

Picokine ELISA Kits

gene name :

IL18

price :

400

assay range :

15.6 pg/ml - 1,000 pg/ml

description :

Rat IL-18 ELISA Kit PicoKine (96 Tests). Quantitate Rat Il18 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.

short description :

Rat IL-18 ELISA Kit PicoKine (96 Tests). Quantitate Rat Il18 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

P97636

immunogen :

Expression system for standard: E.coli; Immunogen sequence: H37-S194

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<1 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

application details :

Dilution ratio of 1:1, concentration in serum is 4-30pg/ml

applications :

ELISA

reactivity :

Rat

images :

/e/k/ek0592_1.png

image labels :

Rat IL-18 PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.027 0.069 0.101 0.173 0.323 0.577 1.092 1.856

created at :

5/17/14 23:17

updated at :

3/2/22 2:54

meta title :

Rat IL-18/IL-1F4/Interleukin-18 ELISA Kit Price PicoKine¬Æ

meta keyword :

Rat IL-18 ELISA Kit PicoKine, Bosterbio High Sensitivity

meta description :

Rat IL-18 ELISA Kit PicoKine (96 Tests). Quantitate Rat Il18 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.

rating value :

99.2

anticoagulant :

heparin or EDTA

assay procedure step 1 :

1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.2 pg/ml, 15.6 pg/ml

background :

Interleukin (IL)-18, also called Interferon-gamma-inducing factor (IGIF), augments natural killer (NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids.1 IL-18 is a recently discovered cytokine that modulates both T helper type 1 (Th1) and Th2 responses.2 IL-18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.3

research category :

Angiogenesis,Cancer,Cancer Metabolism,Cytokines,Immunology,Innate Immunity,Interleukins,Invasion/Microenvironment,Metabolism,Metabolism Processes,Pathways And Processes,Response To Hypoxia,Tumor Immunology

synonyms :

Interleukin-18;IL-18;Interferon gamma-inducing factor;IFN-gamma-inducing factor;Interleukin-1 gamma;IL-1 gamma;Il18;Igif;

gene full name :

Interleukin-18

molecular weight :

22303 MW

protein function :

Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.

subcellular localization :

Secreted.

protein name :

Interleukin-18

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 29 166 511 29 165 490 Standard deviation 1.97 2.2 22.99 2.2 9.07 29.4 CV (%) 6.8% 4.7% 4.5% 7.6% 5.5% 6%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 29 29 30 28 29 0.7 2.4% Sample 2 166 149 165 152 158 7.58 4.7% Sample 3 511 489 520 499 504 11.75 2.3% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

polyclonal antibody from goat polyclonal antibody from goat

normal range citations :

23983360

template :

elisa-kits

more info or order :

Boster product webpage