ELISA/assay

Human VEGF-C ELISA Kit PicoKine®

EK0588

1

Human VEGF-C ELISA Kit PicoKine®

human-vegf-c-picokine-trade-elisa-kit-ek0588-boster

Picokine ELISA Kits

VEGFC

430

62.5 pg/ml - 4,000 pg/ml

Human VEGF-C ELISA Kit PicoKine (96 Tests). Quantitate Human VEGFC in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 3pg/ml.

Human VEGF-C ELISA Kit PicoKine (96 Tests). Quantitate Human VEGFC in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 3pg/ml.

96 wells/kit, with removable strips.

P49767

Expression system for standard: NS0; Immunogen sequence: T103-R227

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<3 pg/ml

10 ng/tube

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Dilution ratio of 1:1, concentration in serum is 500-1250pg/ml

ELISA

Human

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Human VEGF-C PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.110 0.175 0.217 0.298 0.482 0.792 1.413 2.057

5/17/14 23:17

3/2/22 2:54

Human VEGF-C ELISA Kit Price PicoKine® BosterBio

Human VEGF-C ELISA Kit PicoKine, Bosterbio High Sensitivity

Human VEGF-C ELISA Kit PicoKine (96 Tests). Quantitate Human VEGFC in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 3pg/ml.

99.4

heparin or EDTA

4,000 pg/ml,2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml

Vascular endothelial growth factor C is a VEGF. The human gene encoding it is VEGFC. The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family, is active in angiogenesis, lymphangiogenesis and endothelial cell growth and survival, and can also affect the permeability of blood vessels. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-3 receptors. Only the fully processed form can bind and activate VEGFR-2 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor D (VEGF-D). The C terminus of VEGFC has cysteine-rich repeat units characteristic of the Balbiani ring 3 protein (BR3P) of the midge Chironomus tentans.1,2 The standard product used in this kit is recombinant human VEGF-C, consisting of 135 amino acids with the molecular mass of 23Kda after glycosylation.

Angiogenesis,Cancer,Cardiovascular,Growth Factors,Growth Factors/Hormones,Invasion/Microenvironment,Neurogenesis,Neurology Process,Neuroscience,Signal Transduction

Vascular endothelial growth factor C;VEGF-C;Flt4 ligand;Flt4-L;Vascular endothelial growth factor-related protein;VRP;VEGFC;

Vascular endothelial growth factor C

46883 MW

Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors.

Secreted.

Spleen, lymph node, thymus, appendix, bone marrow, heart, placenta, ovary, skeletal muscle, prostate, testis, colon and small intestine and fetal liver, lung and kidney, but not in peripheral blood lymphocyte.

Vascular endothelial growth factor C

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 86 845 1351 89 836 1341 Standard deviation 4.04 56.61 72.95 5.51 71.89 76.43 CV (%) 4.7% 6.7% 5.4% 6.2% 8.6% 5.7%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 86 84 90 88 87 2.23 2.5% Sample 2 845 745 816 748 788 43.24 5.4% Sample 3 1351 1280 1349 1264 1311 39.41 3% *number of samples for each test n=16.

15-25min

monoclonal antibody from mouse polyclonal antibody from goat

22855190

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