Rat TIMP-1 ELISA Kit PicoKine¬Æ | Boster EK0583 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Rat TIMP-1 ELISA Kit PicoKine¬Æ

catalog :

EK0583

more info or order :

Boster product webpage

citations: 1

Reference
Qian Y, Fu X, Hu R, Shen L, Bai H. [Effects of Corbrin Shugan capsule on dimethylnitrosamine-induced hepatic fibrosis in rats]. Zhejiang Da Xue Xue Bao Yi Xue Ban. 2013;42:561-6 pubmed

image

image 1 :

Rat TIMP-1 PicoKine ELISA Kit standard curve

product information

sku :

EK0583

status :

name :

Rat TIMP-1 ELISA Kit PicoKine¬Æ

url key :

rat-timp-1-picokine-trade-elisa-kit-ek0583-boster

product category :

Picokine ELISA Kits

gene name :

TIMP1

price :

390

assay range :

31.2 pg/ml - 2,000 pg/ml

description :

Rat TIMP-1 ELISA Kit PicoKine (96 Tests). Quantitate Rat Timp1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 3pg/ml.

short description :

Rat TIMP-1 ELISA Kit PicoKine (96 Tests). Quantitate Rat Timp1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 3pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

P30120

immunogen :

Expression system for standard: NS0; Immunogen sequence: C24-A217

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<3 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

applications :

ELISA

reactivity :

Rat

images :

/antibody/ek0583-2-ELISA-rat-timp-1-picokine-elisa-kit.jpg

image labels :

Rat TIMP-1 PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.045 0.068 0.094 0.165 0.333 0.706 1.437 2.283

created at :

5/17/14 23:17

updated at :

3/2/22 2:54

meta title :

Rat TIMP-1 ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Rat TIMP-1 ELISA Kit PicoKine, Bosterbio High Sensitivity

meta description :

Rat TIMP-1 ELISA Kit PicoKine (96 Tests). Quantitate Rat Timp1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 3pg/ml.

rating value :

98.7

anticoagulant :

heparin or EDTA

assay procedure step 1 :

2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.2 pg/ml

background :

The tissue inhibitor of metalloproteinases 1 (TIMP1) is also called erythroid-potentiating activity (EPA). The X-linked gene for human TIMP1 is expressed in some but not all inactive X-containing somatic-cell hybrids, suggesting that this gene is either prone to reactivation or variable in its inactivation.1 Purified EPA specifically stimulates human and murine cells of the erythroid lineage, unlike murine interleukin-3 (IL-3) which stimulates precursor cells from all haematopoietic lineages.2 TIMP1 is thought to play a regulatory role in connective tissues by forming inactive complexes with those metalloproteinases that are normally responsible for connective tissue turnover. The human gene encoding TIMP has been mapped to the X chromosome in the region Xp11.1-p11.4.3 The standard product used in this kit is recombinant rat TIMP-1, consisting of 194 amino acids with the molecular mass of 21.5KDa. As a result of glycosylation, the molecular mass is 32-34KDa.

research category :

Invasion/Microenvironment,Protease Inhibitors,Proteolysis / Ubiquitin,Signal Transduction

synonyms :

Metalloproteinase inhibitor 1;Tissue inhibitor of metalloproteinases 1;TIMP-1;Timp1;Timp-1;

gene full name :

Metalloproteinase inhibitor 1

molecular weight :

23794 MW

protein function :

Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Also stimulates steroidogenesis by Leydig and ovarian granuloma cells; procathepsin L is required for maximal activity.

subcellular localization :

Secreted .

protein name :

Metalloproteinase inhibitor 1

sequence similarities :

Belongs to the protease inhibitor I35 (TIMP) family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 59 252 889 61 252 895 Standard deviation 4.24 13.1 45.33 5 13.35 46.54 CV (%) 7.2% 5.2% 5.2%% 8.2% 5.3% 5.2%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 59 60 56 65 60 3.24 5.4% Sample 2 252 245 247 261 251 6.17 2.4% Sample 3 889 980 917 861 911 44.09 4.8% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

normal range citations :

24167139

template :

elisa-kits

more info or order :

Boster product webpage