ELISA/assay

Human EG-VEGF/PK1/PROK1 ELISA Kit PicoKine®

EK0575

1

Human EG-VEGF/PK1/PROK1 ELISA Kit PicoKine®

human-eg-vegf-picokine-trade-elisa-kit-ek0575-boster

Picokine ELISA Kits

PROK1

400

15.6 pg/ml - 1,000 pg/ml

Human EG-VEGF / PK1 ELISA Kit PicoKine (96 Tests). Quantitate Human PROK1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 7pg/ml.

Human EG-VEGF / PK1 ELISA Kit PicoKine (96 Tests). Quantitate Human PROK1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 7pg/ml.

96 wells/kit, with removable strips.

P58294

Expression system for standard: NS0; Immunogen sequence: A20-F105

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<7 pg/ml

10 ng/tube

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Dilution ratio of 1:1, concentration in serum is around 100pg/ml

ELISA

Human

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Human EG-VEGF PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.123 0.167 0.203 0.287 0.468 0.734 1.246 2.050

5/17/14 23:17

3/2/22 2:54

Human EG-VEGF/PK1/PROK1 ELISA Kit Price PicoKine® BosterBio

Human EG-VEGF ELISA Kit, EG VEGF/Prokineticin 1 ELISA

Human EG-VEGF / PK1 ELISA Kit PicoKine (96 Tests). Quantitate Human PROK1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 7pg/ml.

97.9

heparin or EDTA

1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.2 pg/ml, 15.6 pg/ml

EG-VEGF, Endocrine glandderived vascular endothelial growth factor, is a human protein encoded by by the PROKR1 gene. Its gene is mapped to chromosome 1. The 1.4-kb cDNA encodes a protein of 105 amino acids with a well defined signal sequence. The mature protein is predicted to have 86 amino acids. Northern blot analysis demonstrated expression in testis, ovary, adrenal gland, and placenta. EG-VEGF has played a vital role in inducing proliferation, migration, and fenestration in capillary endothelial cells derived from endocrine glands. The standards of this kit are recombinant human EG-VEGF (A20-F105), with molecular weight of 10.5 KDa.

Growth Factors/Hormones,Signal Transduction

Prokineticin-1;Endocrine-gland-derived vascular endothelial growth factor;EG-VEGF;Mambakine;PROK1;UNQ600/PRO1186;

Prokineticin-1

11715 MW

Potently contracts gastrointestinal (GI) smooth muscle. Induces proliferation, migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. Has little or no effect on a variety of other endothelial and non-endothelial cell types. Induces proliferation and differentiation, but not migration, of enteric neural crest cells. Directly influences neuroblastoma progression by promoting the proliferation and migration of neuroblastoma cells. Positively regulates PTGS2 expression and prostaglandin synthesis. May play a role in placentation. May play a role in normal and pathological testis angiogenesis.

Secreted.

Localizes to glandular epithelium, stroma and vascular epithelial cells of first trimester decidua (at protein level). Up-regulated in first trimester decidua when compared with non-pregnant endometrium. Expressed in the steroidogenic glands, ovary, testis, adrenal and placenta.

Prokineticin-1

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 249 474 31 228 490 Standard deviation 1.45 18.17 30.81 1.61 18.92 39.2 CV (%) 4.7% 7.3% 6.5% 5.2% 8.3% 8%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 35 30 33 32 1.92 6% Sample 2 249 238 216 221 231 13.2 5.7% Sample 3 474 409 474 466 455 27.18 5.9% *number of samples for each test n=16.

15-25min

monoclonal antibody from mouse polyclonal antibody from goat

22847371

elisa-kits