Mouse CD40 Ligand/TNFSF5/CD40LG ELISA Kit PicoKine® | Boster EK0574 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse CD40 Ligand/TNFSF5/CD40LG ELISA Kit PicoKine®

catalog :

EK0574

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK0574

Product Name :

Mouse CD40 Ligand/TNFSF5/CD40LG ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Mouse CD40 Ligand/TNFSF5/CD40LG ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cd40lg in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Reactivity :

Mouse

Application :

ELISA

Gene Name :

CD40LG

Uniprot ID :

P27548

Immunogen :

Expression system for standard: NS0; Immunogen sequence: E61-L260

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

62.5 pg/ml - 4,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

monoclonal antibody from rat, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

CD40 ligand

Synonyms :

CD40 ligand;CD40-L;T-cell antigen Gp39;TNF-related activation protein;TRAP;Tumor necrosis factor ligand superfamily member 5;CD154;CD40 ligand, membrane form;CD40 ligand, soluble form;Cd40lg;Cd40l, Tnfsf5;

Protein Name :

CD40 ligand

Molecular Weight :

29370 MW

Protein Function :

Cytokine that binds to TNFRSF5. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching (By similarity).

Subcellular Localization :

Cell membrane; Single-pass type II membrane protein.

Tissue Specificity :

Specifically expressed on activated CD4+ T- lymphocytes.

Background :

CD40 ligand (CD40L) is a type II membrane protein of 261 amino acids on activated T cells that induces B cell proliferation and immunoglobulin secretion. It has homology with tumour necrosis factor-alpha and -beta, and has important functions in B-cell activation and differentiation. Human CD40L with 5 exons, is mapped to the proximal region of the mouse X chromosome on Xq26.3-27.1, and can be detected on T cells but is absent from B cells and monocytes. Since CD40L is expressed on platelets and released from them on activation, its predictive value as a marker for clinical outcome and the therapeutic effect of inhibition of glycoprotein IIb/IIIa receptor in patients with acute coronary syndromes was investigated. The soluble CD40L may be involved in the process of restenosis and that it exerts its effect by triggering a complex group of inflammatory reactions on endothelial and mononuclear cells.CD40L plays a central role in the pathophysiology of acute coronary syndromes, and has a role in the pathogenesis of coronary artery lesions.

Research Category :

Adaptive Immunity, Atherosclerosis, Cardiovascular, Cytokines, Immunoglobulins, Immunology, Innate Immunity, Platelets, T Cells, Tnf Superfamily, Vascular Inflammation