ELISA/assay

Human CD40 Ligand/TNFSF5/CD40LG ELISA Kit PicoKine®

EK0573

1

Human CD40 Ligand/TNFSF5/CD40LG ELISA Kit PicoKine®

human-scd40l-picokine-trade-elisa-kit-ek0573-boster

Picokine ELISA Kits

CD40LG

430

62.5 pg/ml - 4,000 pg/ml

Human CD40 Ligand/TNFSF5/CD40LG ELISA Kit PicoKine (96 Tests). Quantitate Human CD40LG in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml.

Human CD40 Ligand/TNFSF5/CD40LG ELISA Kit PicoKine (96 Tests). Quantitate Human CD40LG in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml.

96 wells/kit, with removable strips.

P29965

Expression system for standard: E.coli; Immunogen sequence: E108-L261

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<15 pg/ml

10 ng/tube

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

ELISA

Human

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Human sCD40L PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 62.5 125 250 500 1,000 2,000 4,000 O.D. 0.025 0.058 0.104 0.163 0.341 0.572 1.122 1.850

5/17/14 23:17

3/2/22 2:54

Human CD40 Ligand/TNFSF5/CD40LG ELISA Kit Price PicoKine®

Human sCD40L ELISA Kit, Bosterbio High Sensitivity

Human CD40 Ligand/TNFSF5/CD40LG ELISA Kit PicoKine (96 Tests). Quantitate Human CD40LG in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml.

98.7

heparin or EDTA

4,000 pg/ml, 2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml

CD40 ligand (CD40L) is a type II membrane protein of 261 amino acids on activated T cells that induces B cell proliferation and immunoglobulin secretion. It has homology with tumour necrosis factor-alpha and -beta, and has important functions in B-cell activation and differentiation. Human CD40L with 5 exons, is mapped to the proximal region of the mouse X chromosome on Xq26.3-27.1, and can be detected on T cells but is absent from B cells and monocytes. Since CD40L is expressed on platelets and released from them on activation, its predictive value as a marker for clinical outcome and the therapeutic effect of inhibition of glycoprotein IIb/IIIa receptor in patients with acute coronary syndromes was investigated. The soluble CD40L may be involved in the process of restenosis and that it exerts its effect by triggering a complex group of inflammatory reactions on endothelial and mononuclear cells.CD40L plays a central role in the pathophysiology of acute coronary syndromes, and has a role in the pathogenesis of coronary artery lesions.

Adaptive Immunity,Atherosclerosis,Cardiovascular,Cytokines,Immunoglobulins,Immunology,Innate Immunity,Platelets,T Cells,Tnf Superfamily,Vascular Inflammation

CD40 ligand;CD40-L;T-cell antigen Gp39;TNF-related activation protein;TRAP;Tumor necrosis factor ligand superfamily member 5;CD154;CD40 ligand, membrane form;CD40 ligand, soluble form;CD40LG;CD40L, TNFSF5, TRAP;

CD40 ligand

29274 MW

Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.

Cell membrane; Single-pass type II membrane protein.

Specifically expressed on activated CD4+ T- lymphocytes.

CD40 ligand

Belongs to the tumor necrosis factor family.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 203 762 1128 198 762 1161 Standard deviation 15.42 58.1%8 56.4 17.02 61.72 77.78 CV (%) 7.6% 7.4% 5% 8.6% 8.1% 6.7%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 203 178 187 209 194 12.35 6.3% Sample 2 762 793 661 730 736 48.95 6.6% Sample 3 1128 1109 1044 1131 1103 35.09 3.1% *number of samples for each test n=16.

15-25min

monoclonal antibody from mouse polyclonal antibody from goat

25648034, 24964251

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