Human E-Cadherin/CDH1/Cadherin-1 ELISA Kit PicoKine¬Æ

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human E-Cadherin/CDH1/Cadherin-1 ELISA Kit PicoKine¬Æ

catalog :

EK0561

more info or order :

Boster product webpage

citations: 2

Reference
Tsalikidis C, Papachristou F, Pitiakoudis M, Asimakopoulos B, Trypsianis G, Bolanaki E, et al. Soluble E-cadherin as a diagnostic and prognostic marker in gastric carcinoma. Folia Med (Plovdiv). 2013;55:26-32 pubmed
Tsai H, Lee T, Yang S, Lin L, Tee Y, Wang P. Markedly elevated soluble E-cadherin in plasma of patient with pelvic inflammatory disease. Fertil Steril. 2013;99:490-5 pubmed publisher

image

image 1 :

Human E-Cadherin PicoKine ELISA Kit standard curve

product information

sku :

EK0561

status :

name :

Human E-Cadherin/CDH1/Cadherin-1 ELISA Kit PicoKine¬Æ

url key :

human-e-cadherin-picokine-trade-elisa-kit-ek0561-boster

product category :

Picokine ELISA Kits

gene name :

CDH1

price :

430

assay range :

156 pg/ml - 10,000 pg/ml

description :

Human E-Cadherin 1 ELISA Kit PicoKine (96 Tests). Quantitate Human CDH1 in cell culture supernatants, serum, plasma (heparin) , saliva and urine. Sensitivity: 15pg/ml.

short description :

Human E-Cadherin 1 ELISA Kit PicoKine (96 Tests). Quantitate Human CDH1 in cell culture supernatants, serum, plasma (heparin) , saliva and urine. Sensitivity: 15pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

P12830

immunogen :

Expression system for standard: NS0; Immunogen sequence: D155-I707

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<15 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, serum, plasma (heparin) , saliva and urine.

applications :

ELISA

reactivity :

Human

images :

/e/k/ek0561.png

image labels :

Human E-Cadherin PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.090 0.133 0.194 0.332 0.538 0.736 1.342 2.038

created at :

5/18/14 6:17

updated at :

3/2/22 2:54

meta title :

Human E-Cadherin/CDH1/Cadherin-1 ELISA Kit Price PicoKine¬Æ

meta keyword :

Human E-Cadherin ELISA Kit PicoKine, Bosterbio High Sensitivity

meta description :

Human E-Cadherin 1 ELISA Kit PicoKine (96 Tests). Quantitate Human CDH1 in cell culture supernatants, serum, plasma (heparin) , saliva and urine. Sensitivity: 15pg/ml.

rating value :

100

anticoagulant :

heparin

assay procedure step 1 :

10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 313 pg/ml, 156 pg/ml

background :

E-Cadherin, also called Cadherin 1 (CDH1), Uvomorulin or calcium-dependent ashesion protein, epithelial. E-cadherin is a Ca (2+)-dependent epithelial cell-cell adhesion molecule. Downregulation of E-cadherin expression often correlates with strong invasive potential and poor prognosis of human carcinomas. The gene spans a region of approximately 100 kb, and its location on chromosome 16q22.1. It contains 16 exons and a 65-kb-long intron 2. E-cadherin gene mutations may contribute to the development of diffusely growing gastric carcinomas. E-cadherin plays a central part in the process of epithelial morphogenesis and acts as a strong invasion suppressor in experimental tumor cell systems. The standard product used in this kit is recombinant gene expression with the molecular mass of 120KDa.

research category :

Cadherins,Calcium Binding Proteins,Calcium Signaling,Cancer,Cell Adhesion,Cytoskeleton / Ecm,Invasion/Microenvironment,Signal Transduction,Signaling Pathway

synonyms :

Cadherin-1;CAM 120/80;Epithelial cadherin;E-cadherin;Uvomorulin;CD324;E-Cad/CTF1;E-Cad/CTF2;E-Cad/CTF3;CDH1;CDHE, UVO;

gene full name :

Cadherin-1

molecular weight :

97456 MW

protein function :

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.

subcellular localization :

Cell junction. Cell membrane; Single-pass type I membrane protein. Endosome. Golgi apparatus, trans-Golgi network. Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma- catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane.

tissue specificity :

Non-neural epithelial tissues.

protein name :

Cadherin-1

sequence similarities :

Contains 5 cadherin domains.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 265 1264 4789 253 1258 4691 Standard deviation 15.37 58.14 324.65 16.69 61.64 318.98 CV (%) 5.8% 4.6% 6.8% 6.6% 4.9% 6.8%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 265 266 300 267 274 14.73 5.3% Sample 2 1264 1074 1156 1112 1151 71.13 6.1% Sample 3 4789 4996 5212 5707 5176 341.11 6.5% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

normal range citations :

24712279, 23122825

template :

elisa-kits

more info or order :

Boster product webpage