ELISA/assay

Human Angiopoietin-1 / ANG1 ELISA Kit PicoKine®

EK0559

1

Human Angiopoietin-1 / ANG1 ELISA Kit PicoKine®

human-angiopoietin-1-picokine-trade-elisa-kit-ek0559-boster

Picokine ELISA Kits

ANGPT1

369

156 pg/ml - 10,000 pg/ml

Human Angiopoietin-1 / ANG1 ELISA Kit PicoKine (96 Tests). Quantitate Human ANGPT1 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and human milk. Sensitivity: 10pg/ml.

Human Angiopoietin-1 / ANG1 ELISA Kit PicoKine (96 Tests). Quantitate Human ANGPT1 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and human milk. Sensitivity: 10pg/ml.

96 wells/kit, with removable strips.

Q15389

Expression system for standard: NS0; Immunogen sequence: H16-F498

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

10 ng/tube

cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and human milk.

Dilution ratio of 1:10, concentration in serum is 20-30ng

ELISA

Human

/antibody/ek0559-2-ELISA-human-angiopoietin-1-picokine-elisa-kit.jpg

Human Angiopoietin-1 PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 156 312 625 125000 25000 5000 10000 O.D. 0.092 0.118 0.233 0.233 0.375 0.684 1.227 2.137

5/18/14 6:17

3/2/22 2:54

Human Angiopoietin-1 / ANG1 ELISA Kit Price PicoKine®

Human Angiopoietin-1 ELISA Kit PicoKine, Bosterbio High Sensitivity

Human Angiopoietin-1 / ANG1 ELISA Kit PicoKine (96 Tests). Quantitate Human ANGPT1 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and human milk. Sensitivity: 10pg/ml.

100

heparin or EDTA

10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 312 pg/ml, 156 pg/ml

Angiopoietin 1, also called ANG1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. This gene was mapped to 8q23.1. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.

Angiogenesis,Cardiovascular,Growth Factors

Angiopoietin-1;ANG-1;ANGPT1;KIAA0003;

Angiopoietin-1

57513 MW

Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme.

Secreted.

Angiopoietin-1

Contains 1 fibrinogen C-terminal domain.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 252 1375 5201 272 1440 5518 Standard deviation 15.37 90.75 358.86 17.68 102.24 452.47 CV (%) 6.1% 6.6% 6.9% 6.5% 7.1% 8.2%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 252 209 251 225 234 18.15 7.7% Sample 2 1375 1410 1341 1498 1406 58.45 4.1% Sample 3 5201 4542 5134 5110 4996 264.65 5.2% *number of samples for each test n=16.

15-25min

monoclonal antibody from mouse polyclonal antibody from goat

23823085, 23094194, 23056802

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