ELISA/assay

Human ACE/Cd143 ELISA Kit PicoKine®

EK0557

1

Human ACE/Cd143 ELISA Kit PicoKine®

human-ace-picokine-trade-elisa-kit-ek0557-boster

Picokine ELISA Kits

ACE

450

0.78 ng/ml - 50 ng/ml

Human ACE/Cd143 ELISA Kit PicoKine (96 Tests). Quantitate Human ACE in cell culture supernatants, serum, plasma (heparin) and saliva. Sensitivity: 5pg/ml.

Human ACE/Cd143 ELISA Kit PicoKine (96 Tests). Quantitate Human ACE in cell culture supernatants, serum, plasma (heparin) and saliva. Sensitivity: 5pg/ml.

96 wells/kit, with removable strips.

P12821

Expression system for standard: NS0; Immunogen sequence: L30-L1261

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<5 pg/ml

50 ng/tube

cell culture supernatants, serum, plasma (heparin) and saliva.

ELISA

Human

/e/k/ek0557_4.png

Human ACE PicoKine ELISA Kit standard curve

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.039 0.099 0.183 0.299 0.512 0.916 1.371 1.923

5/18/14 6:17

3/2/22 2:54

Human ACE/Cd143 ELISA Kit Price PicoKine® BosterBio

Human ACE ELISA Kit, Angiotensin Converting Enzyme 1

Human ACE/Cd143 ELISA Kit PicoKine (96 Tests). Quantitate Human ACE in cell culture supernatants, serum, plasma (heparin) and saliva. Sensitivity: 5pg/ml.

100

heparin

50 ng/ml, 25 ng/ml, 12.5 ng/ml, 6.25 ng/ml, 3.12 ng/ml, 1.56 ng/ml, 0.78 ng/ml

Angiotensin-converting enzyme (ACE) is a zinc-containing dipeptidyl carboxypeptidase widely distributed in mammalian tissues and is thought to play a critical role in blood pressure regulation. The predicted protein is identical, from residue 37 to its C terminus, to the second half or C-terminal domain of the endothelial ACE sequence. The protein sequence inferred consists of a 732-residue preprotein including a 31-residue signal peptide. The mature polypeptide has a molecular weight of 80,073. Although ACE has been studied primarily in the context of its role in blood pressure regulation, this widely distributed enzyme has many other physiological functions. The ACE gene encodes two isozymes. The somatic isozyme is expressed in many tissues, including vascular endothelial cells, renal epithelial cells, and testicular Leydig cells, whereas the testicular or germinal angiotensin-converting enzyme is expressed only in sperm. The standard product used in this kit is recombinant human ACE, consisting of 30-1261 amino acids with the molecular mass of 120KDa.

sc 12184 sc 376132 sc 20791 sc 12187 sc 374198 sc 23909 sc 69992 sc 69991 sc 69993 sc 69994 sc 23908 sc 271860

Cancer,Cancer Metabolism,Cardiovascular,Cell Biology,Cofactors, Vitamins / Minerals,Hematopoietic Progenitors,Metabolism,Metabolism Processes,Metalloprotease,Pathways And Processes,Proteolysis / Ubiquitin,Proteolytic Enzymes,Response To Hypoxia,Serum Proteins,Signal Transduction,Stem Cells,Surface Molecules,Vasculature,Vitamins / Minerals

angiotensin I converting enzyme

53750 MW

Angiotensin-converting enzyme

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1278 6839 24157 1358 6350 25528 Standard deviation 62.62 465.05 1087.06 86.57 482.6 1225.34 CV (%) 4.9% 6.8% 4.5% 6.5% 7.6% 4.8%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1278 1310 1292 1425 1326 58.13 4.3% Sample 2 6839 6994 7039 6669 6885 145.23 2.1% Sample 3 24157 24884 22207 21600 23212 1350.66 5.8% *number of samples for each test n=16.

15-25min

polyclonal antibody from goat polyclonal antibody from goat

20541774

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