Human CCL21 / 6Ckine / Exodus 2 ELISA Kit PicoKine¬Æ

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human CCL21 / 6Ckine / Exodus 2 ELISA Kit PicoKine¬Æ

catalog :

EK0555

more info or order :

Boster product webpage

citations: 2

Reference
Qin Y, He L, Sheng Z, Yong M, Sheng Y, Wei Dong X, et al. Increased CCL19 and CCL21 levels promote fibroblast ossification in ankylosing spondylitis hip ligament tissue. BMC Musculoskelet Disord. 2014;15:316 pubmed publisher
Pranzatelli M, Tate E, McGee N, Ransohoff R. CCR7 signaling in pediatric opsoclonus-myoclonus: upregulated serum CCL21 expression is steroid-responsive. Cytokine. 2013;64:331-6 pubmed publisher

image

image 1 :

Human CCL21/6Ckine PicoKine ELISA Kit standard curve

product information

sku :

EK0555

status :

name :

Human CCL21 / 6Ckine / Exodus 2 ELISA Kit PicoKine¬Æ

url key :

human-ccl21-6ckine-picokine-trade-elisa-kit-ek0555-boster

product category :

Picokine ELISA Kits

gene name :

CCL21

price :

369

assay range :

62.5 pg/ml - 4,000 pg/ml

description :

Human CCL21 / 6Ckine / Exodus 2 ELISA Kit PicoKine (96 Tests). Quantitate Human CCL21 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml.

short description :

Human CCL21 / 6Ckine / Exodus 2 ELISA Kit PicoKine (96 Tests). Quantitate Human CCL21 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

O00585

immunogen :

Expression system for standard: E.coli; Immunogen sequence: S24-P134

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<12 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

application details :

Dilution ratio of 1:1, concentration in serum is around 500pg/ml

applications :

ELISA

reactivity :

Human

images :

/e/k/ek0555.png

image labels :

Human CCL21/6Ckine PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.004 0.036 0.067 0.254 0.396 0.970 1.607 2.207

created at :

7/16/14 6:17

updated at :

3/2/22 2:54

meta title :

Human CCL21 / 6Ckine / Exodus 2 ELISA Kit Price PicoKine¬Æ

meta keyword :

Human CCL21/6Ckine ELISA Kit, ECL ELISA Kit, Bosterbio High Sensitivity

meta description :

Human CCL21 / 6Ckine / Exodus 2 ELISA Kit PicoKine (96 Tests). Quantitate Human CCL21 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml.

rating value :

99.1

anticoagulant :

heparin or EDTA

assay procedure step 1 :

4,000 pg/ml, 2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml

background :

Chemokine (C-C motif) ligand 21 (CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine (because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine (SLC). By somatic cell hybrid and radiation hybrid analyses, mapped the SCYA21 gene to 9p13. Chemokines are a family of proteins that direct leukocyte migration and activation to inflammatory stimuli. CXC chemokine ligand 13 (CXCL13), CC chemokine ligand 21 (CCL21), and CCL19 are constitutively expressed in secondary lymphoid organs, where they control the placement of lymphocytes and dendritic cells. It was demonstrate that the local expression of homeostatic chemokines in nonlymphoid organs, such as the lung, plays an important role in protective immune responses.

research category :

Chemokines,Immunology,Innate Immunity

synonyms :

C-C motif chemokine 21;6Ckine;Beta-chemokine exodus-2;Secondary lymphoid-tissue chemokine;SLC;Small-inducible cytokine A21;CCL21;SCYA21;UNQ784/PRO1600;

gene full name :

C-C motif chemokine 21

molecular weight :

14646 MW

protein function :

Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.

subcellular localization :

Secreted.

tissue specificity :

Highly expressed in high endothelial venules of lymph nodes, spleen and appendix. Intermediate levels found in small intestine, thyroid gland and trachea. Low level expression in thymus, bone marrow, liver, and pancreas. Also found in tonsil, fetal heart and fetal spleen.

protein name :

C-C motif chemokine 21

sequence similarities :

Belongs to the intercrine beta (chemokine CC) family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 100 709 1352 103 706 1475 Standard deviation 4 28.36 82.47 6.9 31.77 92.92 CV (%) 4% 4% 6.1% 6.7% 4.5% 6.3%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 100 109 108 95 103 5.78 5.6% Sample 2 709 697 706 724 709 9.72 1.3% Sample 3 1352 1517 1447 1423 1434 58.95 4.1% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

normal range citations :

25260647, 23764550

template :

elisa-kits

more info or order :

Boster product webpage