ELISA/assay

Human VEGFR2 / KDR / VEGF Receptor 2 ELISA Kit PicoKine®

EK0544

1

Human VEGFR2 / KDR / VEGF Receptor 2 ELISA Kit PicoKine®

human-vegfr2-kdr-picokine-trade-elisa-kit-ek0544-boster

Picokine ELISA Kits

KDR

400

62.5 pg/ml - 4,000 pg/ml

Human VEGFR2 / KDR / VEGF Receptor 2 ELISA Kit PicoKine (96 Tests). Quantitate Human KDR in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 4pg/ml.

Human VEGFR2 / KDR / VEGF Receptor 2 ELISA Kit PicoKine (96 Tests). Quantitate Human KDR in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 4pg/ml.

96 wells/kit, with removable strips.

P35968

Expression system for standard: NS0; Immunogen sequence: A20-E764

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<4 pg/ml

10 ng/tube

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

ELISA

Human

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Human VEGFR2/KDR PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.065 0.115 0.165 0.265 0.446 0.814 1.517 2.462

7/16/14 6:17

3/2/22 2:54

Human VEGFR2 / KDR / VEGF Receptor 2 ELISA Kit Price PicoKine®

Human VEGFR2/KDR ELISA Kit, VEGF Receptor 2 ELISA Kit

Human VEGFR2 / KDR / VEGF Receptor 2 ELISA Kit PicoKine (96 Tests). Quantitate Human KDR in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 4pg/ml.

97.7

heparin or EDTA

4,000 pg/ml, 2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml

Vascular endothelial growth factor receptor 2 (VEGFR-2) also known as Kinase insert domain receptor (KDR, a type III receptor tyrosine kinase) is a VEGF receptor. Through in situ hybridization of a genomic DNA probe to metaphase chromosomes, VEGFR2 was localized to 4q11-->q12. VEGF receptor 2 and the adherens junction act as shear-stress cotransducers, mediating the transduction of shear-stress signals into vascular endothelial cells.

Angiogenesis,Cancer,Cancer Metabolism,Cardiovascular,Growth Factors,Growth Factors/Hormones,Hematopoietic Progenitors,Host Virus Interaction,Interspecies Interaction,Invasion/Microenvironment,Metabolism,Metabolism Processes,Microbiology,Oncoproteins,Oncoproteins/Suppressors,Pathways And Processes,Protein Phosphorylation,Receptor Tyrosine Kinases,Response To Hypoxia,Signal Transduction,Stem Cells,Surface Molecules,Tyrosine Kinases

Vascular endothelial growth factor receptor 2;VEGFR-2;2.7.10.1;Fetal liver kinase 1;FLK-1;Kinase insert domain receptor;KDR;Protein-tyrosine kinase receptor flk-1;CD309;KDR;FLK1, VEGFR2;

Vascular endothelial growth factor receptor 2

151527 MW

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2 and isoform 3, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC.

Cell junction . Endoplasmic reticulum . Localized with RAP1A at cell-cell junctions (By similarity). Colocalizes with ERN1 and XBP1 in the endoplasmic reticulum in endothelial cells in a vascular endothelial growth factor (VEGF)-dependent manner (PubMed:23529610).

Detected in cornea (at protein level). Widely expressed.

Vascular endothelial growth factor receptor 2

Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 177 400 1762 167 410 1810 Standard deviation 10.79 26 137.43 11.52 29.52 161.09 CV (%) 6.1% 6.5% 7.8% 6.9% 7.2% 8.9%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 177 180 169 166 173 5.7 3.2% Sample 2 400 400 444 444 422 22 5.2% Sample 3 1762 1652 1818 1564 1699 98.18 5.7% *number of samples for each test n=16.

15-25min

monoclonal antibody from mouse polyclonal antibody from goat

19551437

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