ELISA/assay

Human sVEGFR1/sFLT1 ELISA Kit PicoKine®

EK0543

1

Human sVEGFR1/sFLT1 ELISA Kit PicoKine®

human-svegfr1-sflt1-picokine-trade-elisa-kit-ek0543-boster

Picokine ELISA Kits

FLT1

370

156 pg/ml - 10,000 pg/ml

Human sVEGFR1/sFLT1 ELISA Kit PicoKine (96 Tests). Quantitate Human FLT1 in cell culture supernatants, serum and plasma (EDTA). Sensitivity: 30pg/ml.

Human sVEGFR1/sFLT1 ELISA Kit PicoKine (96 Tests). Quantitate Human FLT1 in cell culture supernatants, serum and plasma (EDTA). Sensitivity: 30pg/ml.

96 wells/kit, with removable strips.

P17948

Expression system for standard: NS0; Immunogen sequence: S27-H687

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<30 pg/ml

10 ng/tube

cell culture supernatants, serum and plasma (EDTA).

Dilution ratio of 1:1, concentration in serum is 3.6-5ng/ml target in serum is below detection limit.

ELISA

Human

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Human sVEGFR1/sFLT1 PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.031 0.069 0.124 0.164 0.366 0.681 1.178 1.920

5/18/14 6:17

3/2/22 2:54

Human sVEGFR1/sFLT1 ELISA Kit Price PicoKine® BosterBio

Human sVEGFR1/sFLT1 ELISA Kit, Bosterbio High Sensitivity

Human sVEGFR1/sFLT1 ELISA Kit PicoKine (96 Tests). Quantitate Human FLT1 in cell culture supernatants, serum and plasma (EDTA). Sensitivity: 30pg/ml.

100

EDTA

10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 312 pg/ml, 156 pg/ml

sVEGFR1, also known as sFMS-related tyrosine kinase 1 (sFLT1). Oncogene sFLT belongs to the src gene family and is related to oncogene ROS . Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. sFLT is mapped to 13q12. sVEGF receptor 1 signaling is essential for osteoclast development and bone marrow formation in colony-stimulating factor 1-deficient mice. The standard product used in this kit is recombinant human sVEGFR1, consisting of 905 amino acids with the molecular mass of 100KDa.

Angiogenesis,Cancer,Cancer Metabolism,Cardiovascular,Growth Factors,Invasion/Microenvironment,Metabolism,Metabolism Processes,Pathways And Processes,Protein Phosphorylation,Response To Hypoxia,Signal Transduction,Tyrosine Kinases

Vascular endothelial growth factor receptor 1;VEGFR-1;2.7.10.1;Fms-like tyrosine kinase 1;FLT-1;Tyrosine-protein kinase FRT;Tyrosine-protein kinase receptor FLT;FLT;Vascular permeability factor receptor;FLT1;FLT, FRT, VEGFR1;

Vascular endothelial growth factor receptor 1

150769 MW

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts (in vitro). Has very high affinity for VEGFA and relatively low protein kinase activity; may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Likewise, isoforms lacking a transmembrane domain, such as isoform 2, isoform 3 and isoform 4, may function as decoy receptors for VEGFA. Modulates KDR signaling by forming heterodimers with KDR. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Phosphorylates SRC and YES1, and may also phosphorylate CBL. Isoform 1 phosphorylates PLCG. Promotes phosphorylation of AKT1 at 'Ser-473'. Promotes phosphorylation of PTK2/FAK1. Isoform 7 has a truncated kinase domain; it increases phosphorylation of SRC at 'Tyr-418' by unknown means and promotes tumor cell invasion.

Isoform 1: Cell membrane; Single-pass type I membrane protein. Endosome. Autophosphorylation promotes ubiquitination and endocytosis.

Detected in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. Isoform 2 is strongly expressed in placenta. Isoform 3 is expressed in corneal epithelial cells (at protein level). Isoform 3 is expressed in vascular smooth muscle cells (VSMC).

Vascular endothelial growth factor receptor 1

Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 237 1369 4420 240 1352 4316 Standard deviation 9.71 106.78 203.32 11.28 131.14 202.85 CV (%) 4.1% 7.8% 4.6% 4.7% 9.7% 4.7%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 237 212 228 228 226 9.01 3.9% Sample 2 1369 1318 1326 1352 1341 20.36 1.5% Sample 3 4420 4343 4598 4779 4535 168.51 3.7% *number of samples for each test n=16.

15-25min

monoclonal antibody from mouse polyclonal antibody from goat

16449946

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