Mouse VEGF ELISA Kit PicoKine¬Æ | Boster EK0541 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse VEGF ELISA Kit PicoKine¬Æ

catalog :

EK0541

more info or order :

Boster product webpage

citations: 1

Reference
Gu J, Young E, Patterson S, Makey K, Wells J, Huang M, et al. Postmenopausal obesity promotes tumor angiogenesis and breast cancer progression in mice. Cancer Biol Ther. 2011;11:910-7 pubmed

image

image 1 :

Mouse VEGF PicoKine ELISA Kit standard curve

product information

sku :

EK0541

status :

name :

Mouse VEGF ELISA Kit PicoKine¬Æ

url key :

mouse-vegf-picokine-trade-elisa-kit-ek0541-boster

product category :

Picokine ELISA Kits

gene name :

VEGFA

price :

369

assay range :

15.6 pg/ml - 1,000 pg/ml

description :

Mouse VEGF ELISA Kit PicoKine (96 Tests). Quantitate Mouse Vegfa in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 2pg/ml.

short description :

Mouse VEGF ELISA Kit PicoKine (96 Tests). Quantitate Mouse Vegfa in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 2pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

Q00731

immunogen :

Expression system for standard: NS0; Immunogen sequence: A27-R190

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<2 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

applications :

ELISA

reactivity :

Mouse

images :

/e/k/ek0541.png

image labels :

Mouse VEGF PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.035 0.074 0.131 0.211 0.353 0.660 1.201 1.936

created at :

5/18/14 6:17

updated at :

3/2/22 2:54

description before attributes :

Featured Product . Fast ELISA kit now available: Boster is proud to announce the newest Picokine Fast ELISA kits. Now you can perform what used to be 4+ hour ELISA assay in under 1.5 hours. Available for Mouse VEGF PicoKine Fast ELISA Kit and many others.

meta title :

Mouse VEGF ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Mouse VEGF ELISA Kit, VEGF A ELISA Kit, Bosterbio High Sensitivity

meta description :

Mouse VEGF ELISA Kit PicoKine (96 Tests). Quantitate Mouse Vegfa in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 2pg/ml.

rating value :

100

anticoagulant :

heparin, EDTA or citrate

assay procedure step 1 :

1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.3 pg/ml, 15.6 pg/ml

background :

Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis. VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy. VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1.The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12. The standard product used in this kit is recombinant mouse VEGF164, a 42KDa dimer.

research category :

Angiogenesis,Cancer,Cancer Metabolism,Cardiovascular,Growth Factors,Growth Factors/Hormones,Metabolism,Metabolism Processes,Pathways And Processes,Response To Hypoxia,Signal Transduction

synonyms :

Vascular endothelial growth factor A;VEGF-A;Vascular permeability factor;VPF;Vegfa;Vegf;

gene full name :

Vascular endothelial growth factor A

molecular weight :

25283 MW

protein function :

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis (By similarity).

subcellular localization :

Isoform VEGF-1: Secreted.

tissue specificity :

In developing embryos, expressed mainly in the choroid plexus, paraventricular neuroepithelium, placenta and kidney glomeruli. Also found in bronchial epithelium, adrenal gland and in seminiferous tubules of testis. High expression of VEGF continues in kidney glomeruli and choroid plexus in adults.

protein name :

Vascular endothelial growth factor A

sequence similarities :

Belongs to the PDGF/VEGF growth factor family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 40 179 538 40 171 574 Standard deviation 1.92 9.48 36.58 2.6 9.57 48.79 CV (%) 4.8% 5.3% 6.8% 6.5% 5.6% 8.5%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 40 34 35 37 36 2.29 6.3% Sample 2 179 204 186 197 191 9.65 5% Sample 3 538 463 494 471 491 29.15 5.9% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

monoclonal antibody from rat polyclonal antibody from goat

normal range citations :

21451264

template :

elisa-kits

more info or order :

Boster product webpage