Rat VEGF ELISA Kit PicoKine¬Æ | Boster EK0540 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Rat VEGF ELISA Kit PicoKine¬Æ

catalog :

EK0540

more info or order :

Boster product webpage

citations: 1

Reference
Guo W, Li J, Chen Z, Zhuang J, Gu W, Zhang L, et al. Transient increased expression of VEGF and MMP-1 in a rat liver tumor model after hepatic arterial occlusion. Hepatogastroenterology. 2004;51:381-6 pubmed

image

image 1 :

Rat VEGF PicoKine ELISA Kit standard curve

product information

sku :

EK0540

status :

name :

Rat VEGF ELISA Kit PicoKine¬Æ

url key :

rat-vegf-picokine-trade-elisa-kit-ek0540-boster

product category :

Picokine ELISA Kits

gene name :

VEGFA

price :

369

assay range :

15.6 pg/ml - 1,000 pg/ml

description :

Rat VEGF ELISA Kit PicoKine (96 Tests). Quantitate Rat Vegfa in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml.

short description :

Rat VEGF ELISA Kit PicoKine (96 Tests). Quantitate Rat Vegfa in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

B5DEK7

immunogen :

Expression system for standard: NS0; Immunogen sequence: A27-R190

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<1 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

applications :

ELISA

reactivity :

Rat

images :

/e/k/ek0540_1.png

image labels :

Rat VEGF PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.045 0.065 0.128 0.212 0.343 0.660 1.159 1.889

created at :

5/18/14 6:17

updated at :

3/2/22 2:54

meta title :

Rat VEGF ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Rat VEGF ELISA Kit, VEGF A ELISA Kit, Bosterbio High Sensitivity

meta description :

Rat VEGF ELISA Kit PicoKine (96 Tests). Quantitate Rat Vegfa in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml.

rating value :

100

anticoagulant :

heparin, EDTA or citrate

assay procedure step 1 :

1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.3 pg/ml, 15.6 pg/ml

background :

Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis. VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy. VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1. The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12.The standard product used in this kit is recombinant rat VEGF164, which is a 25KDa single chain as well as a 50KDa dimer.

research category :

Angiogenesis,Cancer,Cancer Metabolism,Cardiovascular,Growth Factors,Growth Factors/Hormones,Metabolism,Metabolism Processes,Pathways And Processes,Response To Hypoxia,Signal Transduction

synonyms :

Vascular endothelial growth factor A ;Vegfa ;

gene full name :

vascular endothelial growth factor A

molecular weight :

40241 MW

protein name :

Vascular endothelial growth factor A

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 29 243 389 31 234 368 Standard deviation 1.91 18.95 16.72 2.07 16.75 17.66 CV (%) 6.6% 7.8% 4.3% 6.7% 7.8% 4.8%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 29 28 31 28 29 1.22 4.2% Sample 2 243 226 269 260 249 16.46 6.6% Sample 3 389 381 403 431 401 19.02 4.7% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

normal range citations :

15086165

template :

elisa-kits

more info or order :

Boster product webpage