ELISA/assay

Human VEGF ELISA Kit PicoKine®

EK0539

1

Human VEGF ELISA Kit PicoKine®

human-vegf-picokine-trade-elisa-kit-ek0539-boster

Picokine ELISA Kits

VEGFA

369

31.2 pg/ml - 2,000 pg/ml

Human VEGF ELISA Kit PicoKine (96 Tests). Quantitate Human VEGFA in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml.

Human VEGF ELISA Kit PicoKine (96 Tests). Quantitate Human VEGFA in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml.

96 wells/kit, with removable strips.

P15692

Expression system for standard: NS0; Immunogen sequence: A27-R191

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

There is no detectable cross-reactivity with other relevant proteins.

<1 pg/ml

10 ng/tube

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Dilution ratio of 1:1, concentration in serum is around 80pg/ml

ELISA

Human

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Human VEGF PicoKine ELISA Kit standard curve

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.020 0.055 0.113 0.189 0.345 0.589 1.106 1.913

5/18/14 13:17

3/2/22 2:54

Human VEGF ELISA Kit Price PicoKine® BosterBio

Human VEGF ELISA Kit, VEGF A ELISA Kit, Bosterbio High Sensitivity

Human VEGF ELISA Kit PicoKine (96 Tests). Quantitate Human VEGFA in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml.

99.2

heparin, EDTA or citrate

2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.2 pg/ml

Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis.VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy.VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1. The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12.

Angiogenesis,Cancer,Cancer Metabolism,Cardiovascular,Growth Factors,Growth Factors/Hormones,Metabolism,Metabolism Processes,Pathways And Processes,Response To Hypoxia,Signal Transduction

Vascular endothelial growth factor A;VEGF-A;Vascular permeability factor;VPF;VEGFA;VEGF;

Vascular endothelial growth factor A

27042 MW

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

Secreted . VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.

Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. A higher level expression seen in pituitary tumors as compared to the pituitary gland.

Vascular endothelial growth factor A

Belongs to the PDGF/VEGF growth factor family.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 72 270 946 76 254 955 Standard deviation 4.53 21.33 59.59 9.62 24.38 74.49 CV (%) 9.6% 7.9% 9.6% 7.8% 9.6% 7.8%

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 72 80 82 71 76 4.81 6.3% Sample 2 270 267 252 245 258 10.35 4% Sample 3 946 897 1026 1037 976 57.79 5.9% *number of samples for each test n=16.

15-25min

monoclonal antibody from mouse polyclonal antibody from goat

25374209

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