Mouse VCAM-1 ELISA Kit PicoKine® | Boster EK0538 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse VCAM-1 ELISA Kit PicoKine®

catalog :

EK0538

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK0538

Product Name :

Mouse VCAM-1 ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Mouse VCAM-1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Vcam1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.

Reactivity :

Mouse

Application :

ELISA

Gene Name :

VCAM1

Uniprot ID :

P29533

Immunogen :

Expression system for standard: NS0; Immunogen sequence: F25-E698

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

156 pg/ml - 10,000 pg/ml

Sensitivity :

<5 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

monoclonal antibody from rat, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Vascular cell adhesion protein 1

Synonyms :

Vascular cell adhesion protein 1;V-CAM 1;VCAM-1;CD106;Vcam1;Vcam-1;

Protein Name :

Vascular cell adhesion protein 1

Molecular Weight :

81317 MW

Protein Function :

Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.

Subcellular Localization :

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Tissue Specificity :

Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue. Expressed in the bone marrow.

Sequence Similarities :

Contains 7 Ig-like C2-type (immunoglobulin-like) domains.

Background :

Vascular cell adhesion molecule 1 (VCAM-1) is a cell surface glycoprotein adhesive for certain blood leukocytes and tumor cells, which is expressed by activated endothelium in a variety of pathologic conditions including atherosclerosis.Increased expression of VCAM1 is associated with a variety of chronic inflammatory conditions, making its expression and function a target for therapeutic intervention.Integrin alpha4beta1 (VLA-4) and VCAM-1 facilitate a critical cell-cell adhesion event required for survival of endothelial and mural cells during vascularization.The VCAM1 gene is assigned to the 1p31-32 region of chromosome 1 based on the analysis of human-mouse hybrid cell lines and in situ hybridization.The standard product used in this kit is recombinant mouse VCAM-1, a dimeric protein linking with a disulfide bond. It consists of Phe25-Glu698 amino acid sequence of a single chain with the molecular mass of 101.9KDa. As a result of glycosylation, the molecular mass of 120KDa is revealed by SDS-PAGE.

Research Category :

Atherosclerosis, Cancer, Cardiovascular, Cell Adhesion, Cell Adhesion Molecules, Cytoskeleton / Ecm, Invasion/Microenvironment, Mesenchymal Stem Cells, Neural Signal Transduction, Neurology Process, Neuroscience, Signal Transduction, Stem Cells, Surface Molecules, Vascular Inflammation