Human VCAM-1 ELISA Kit PicoKine¬Æ | Boster EK0537 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human VCAM-1 ELISA Kit PicoKine¬Æ

catalog :

EK0537

more info or order :

Boster product webpage

citations: 1

Reference
Liu R, Wu Y, Fan H, Xu X, Luo Y, Hu J. [Serum levels of sVCAM-1, IL-18 and VEGF in patients with aplastic anemia and their clinical significance]. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2009;17:117-20 pubmed

image

image 1 :

Human VCAM-1 PicoKine ELISA Kit standard curve

product information

sku :

EK0537

status :

name :

Human VCAM-1 ELISA Kit PicoKine¬Æ

url key :

human-vcam-1-picokine-trade-elisa-kit-ek0537-boster

product category :

Picokine ELISA Kits

gene name :

VCAM1

price :

369

assay range :

156 pg/ml - 10,000 pg/ml

description :

Human VCAM-1 ELISA Kit PicoKine (96 Tests). Quantitate Human VCAM1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

short description :

Human VCAM-1 ELISA Kit PicoKine (96 Tests). Quantitate Human VCAM1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

P19320

immunogen :

Expression system for standard: NS0; Immunogen sequence: F25-E698

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<10 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

application details :

Dilution ratio of 1:300, concentration in serum is around 500ng/ml

applications :

ELISA

reactivity :

Human

images :

/e/k/ek0537.png

image labels :

Human VCAM-1 PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.065 0.149 0.183 0.291 0.487 0.763 1.141 1.803

created at :

5/18/14 13:17

updated at :

3/2/22 2:54

meta title :

Human VCAM-1 ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Human VCAM-1 ELISA Kit PicoKine, Bosterbio High Sensitivity

meta description :

Human VCAM-1 ELISA Kit PicoKine (96 Tests). Quantitate Human VCAM1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

rating value :

99.4

anticoagulant :

heparin or EDTA

assay procedure step 1 :

10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 313 pg/ml, 156 pg/ml

background :

Vascular cell adhesion molecule 1 (VCAM-1) is a cell surface glycoprotein adhesive for certain blood leukocytes and tumor cells, which is expressed by activated endothelium in a variety of pathologic conditions including atherosclerosis. Increased expression of VCAM1 is associated with a variety of chronic inflammatory conditions, making its expression and function a target for therapeutic intervention. Integrin alpha4beta1 (VLA-4) and VCAM-1 facilitate a critical cell-cell adhesion event required for survival of endothelial and mural cells during vascularization. The VCAM1 gene is assigned to the 1p31-32 region of chromosome 1 based on the analysis of human-mouse hybrid cell lines and in situ hybridization. The standard product used in this kit is recombinant human VCAM-1, consisting of Phe25-Glu698 amino acid sequence of a single chain with the molecular mass of 74KDa. As a result of glycosylation, the molecular mass of 90-95KDa is revealed by SDS-PAGE.

research category :

Atherosclerosis,Cancer,Cardiovascular,Cell Adhesion,Cell Adhesion Molecules,Cytoskeleton / Ecm,Invasion/Microenvironment,Mesenchymal Stem Cells,Neural Signal Transduction,Neurology Process,Neuroscience,Signal Transduction,Stem Cells,Surface Molecules,Vascular Inflammation

synonyms :

Vascular cell adhesion protein 1;V-CAM 1;VCAM-1;INCAM-100;CD106;VCAM1;L1CAM;

gene full name :

Vascular cell adhesion protein 1

molecular weight :

81276 MW

protein function :

Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.

subcellular localization :

Membrane; Single-pass type I membrane protein.

tissue specificity :

Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.

protein name :

Vascular cell adhesion protein 1

sequence similarities :

Contains 7 Ig-like C2-type (immunoglobulin-like) domains.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 310 1065 5287 304 1029 5603 Standard deviation 17.67 51.12 387.35 19.15 65.85 481.85 CV (%) 5.7% 4.8% 7.3% 6.4% 6.4% 8.6%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 310 273 296 273 288 15.79 5.4% Sample 2 1065 1111 1014 939 1032 63.84 6.1% Sample 3 5287 5539 5884 6073 5695 303.89 5.3% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

normal range citations :

19236760

template :

elisa-kits

more info or order :

Boster product webpage