Human uPAR / URKR / uPA Receptor ELISA Kit PicoKine¬Æ

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human uPAR / URKR / uPA Receptor ELISA Kit PicoKine¬Æ

catalog :

EK0536

more info or order :

Boster product webpage

citations: 1

Reference
Jing J, Zheng S, Han C, Du L, Guo Y, Wang P. Evaluating the value of uPAR of serum and tissue on patients with cervical cancer. J Clin Lab Anal. 2012;26:16-21 pubmed publisher

image

image 1 :

Human uPAR PicoKine ELISA Kit standard curve

product information

sku :

EK0536

status :

name :

Human uPAR / URKR / uPA Receptor ELISA Kit PicoKine¬Æ

url key :

human-upar-picokine-trade-elisa-kit-ek0536-boster

product category :

Picokine ELISA Kits

gene name :

PLAUR

price :

370

assay range :

62.5 pg/ml - 4,000 pg/ml

description :

Human uPAR / URKR / uPA Receptor ELISA Kit PicoKine (96 Tests). Quantitate Human PLAUR in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 4pg/ml.

short description :

Human uPAR / URKR / uPA Receptor ELISA Kit PicoKine (96 Tests). Quantitate Human PLAUR in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 4pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

Q03405

immunogen :

Expression system for standard: NS0; Immunogen sequence: L23-R303

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<4 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

applications :

ELISA

reactivity :

Human

images :

/e/k/ek0536.png

image labels :

Human uPAR PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.025 0.085 0.164 0.345 0.602 1.078 1.536 2.011

created at :

5/18/14 13:17

updated at :

3/2/22 2:54

meta title :

Human uPAR / URKR / uPA Receptor ELISA Kit Price PicoKine¬Æ

meta keyword :

Human uPAR ELISA Kit, uPA Receptor/uPAR ELISA, Bosterbio High Sensitivity

meta description :

Human uPAR / URKR / uPA Receptor ELISA Kit PicoKine (96 Tests). Quantitate Human PLAUR in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 4pg/ml.

rating value :

98.8

anticoagulant :

heparin or EDTA

assay procedure step 1 :

4,000 pg/ml, 2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml

background :

The urokinase-type plasminogen activator receptor (uPAR) is a key molecule in the regulation of cell-surface plasminogen activation and, as such, plays an important role in many normal as well as pathological processes. The cDNA for Mo3, an activation antigen expressed by human monocytes and myelomonocytic cell lines after stimulation by a variety of agents. Mo3 expression in vivo is associated predominantly with macrophages in inflammatory sites. It is a highly glycosylated protein of about 50 kD in monocytes where it is anchored to the plasma membrane by glycosyl-phosphatidylinositol linkage. The complete coding sequence of the cDNA has been found to encode 335 amino acids including a predicted signal peptide of 22 residues and a hydrophobic C-terminal portion. Mo3 is identical to the human receptor for the urokinase plasminogen activator. UPAR is a useful prognostic marker for biologically aggressive forms of endometrial cancer. PLAUR is located at chromosome 19q13.1-q13.2. The standard product used in this kit is recombinant human uPAR, consisting of 287 amino acids with the molecular mass of 31KDa.

research category :

Cancer,Cardiovascular,Cell Type Markers,Extracellular Matrix,Immunology,Invasion/Microenvironment,Receptors,Tumor Biomarkers

synonyms :

Urokinase plasminogen activator surface receptor;U-PAR;uPAR;Monocyte activation antigen Mo3;CD87;PLAUR;MO3, UPAR;

gene full name :

Urokinase plasminogen activator surface receptor

molecular weight :

36978 MW

protein function :

Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form.

subcellular localization :

Cell membrane . Cell projection, invadopodium membrane . Colocalized with FAP (seprase) preferentially at the cell surface of invadopodia membrane in a cytoskeleton-, integrin- and vitronectin-dependent manner.

tissue specificity :

Expressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain.

protein name :

Urokinase plasminogen activator surface receptor

sequence similarities :

Contains 3 UPAR/Ly6 domains.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 162 487 1379 146 478 1361 Standard deviation 10.69 37.49 66.19 12.11 37.28 85.74 CV (%) 6.6% 7.7% 4.8% 8.3% 7.8% 6.3%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 162 174 158 168 165 6.06 3.6% Sample 2 487 514 466 509 494 19.09 3.8% Sample 3 1379 1232 1375 1306 1323 60.02 4.5% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

polyclonal antibody from goat polyclonal antibody from goat

normal range citations :

24833530

template :

elisa-kits

more info or order :

Boster product webpage