Human uPA / PLAU / URK ELISA Kit PicoKine¬Æ | Boster EK0535 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human uPA / PLAU / URK ELISA Kit PicoKine¬Æ

catalog :

EK0535

more info or order :

Boster product webpage

citations: 1

Reference
Wang X, Zhou Z, Xu Y. [Changes of plasma uPA and TGF-beta1 in patients with preeclampsia]. Sichuan Da Xue Xue Bao Yi Xue Ban. 2010;41:118-20 pubmed

image

image 1 :

Human uPA/PLAU PicoKine ELISA Kit standard curve

product information

sku :

EK0535

status :

name :

Human uPA / PLAU / URK ELISA Kit PicoKine¬Æ

url key :

human-upa-plau-picokine-trade-elisa-kit-ek0535-boster

product category :

Picokine ELISA Kits

gene name :

PLAU

price :

370

assay range :

62.5 pg/ml - 4,000 pg/ml

description :

Human uPA / PLAU / URK ELISA Kit PicoKine (96 Tests). Quantitate Human PLAU in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.

short description :

Human uPA / PLAU / URK ELISA Kit PicoKine (96 Tests). Quantitate Human PLAU in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

P00749

immunogen :

Expression system for standard: NS0; Immunogen sequence: M1-L431

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<5 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

application details :

Dilution ratio of 1:1, concentration in serum is 1000pg/ml 2000pg/ml

applications :

ELISA

reactivity :

Human

images :

/e/k/ek0535_1.png

image labels :

Human uPA/PLAU PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.010 0.089 0.139 0.250 0.693 1.114 1.781 2.050

created at :

5/18/14 13:17

updated at :

3/2/22 2:54

meta title :

Human uPA / PLAU / URK ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Human uPA/PLAU ELISA Kit, Bosterbio High Sensitivity

meta description :

Human uPA / PLAU / URK ELISA Kit PicoKine (96 Tests). Quantitate Human PLAU in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.

rating value :

100

anticoagulant :

heparin or EDTA

assay procedure step 1 :

4,000 pg/ml, 2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml

background :

Plasminogen activator,urokinase (PLAU, uPA) converts plasminogen to plasmin. Plasmin is involved in processing of amyloid precursor protein and degrades secreted and aggregated amyloid-beta, a hallmark of Alzheimer disease (AD). Urokinase has a molecular mass of about 54 kD and is composed of 2 disulfide-linked chains, A and B, of molecular masses 18 kD and 33 kD, respectively. It localized on 10q24. uPA facilitates cell migration by localizing proteolisys on the cell surface and by inducing intracellular signalling pathways. In human vascular smooth muscle cell (VSMC), uPA stimulates migration via the uPA receptor (uPAR) signalling complex containing TYK2 and phosphatidylinositol 3-kinase (PI3-K).

synonyms :

Urokinase-type plasminogen activator;U-plasminogen activator;uPA;3.4.21.73;Urokinase-type plasminogen activator long chain A;Urokinase-type plasminogen activator short chain A;Urokinase-type plasminogen activator chain B;PLAU;

gene full name :

Urokinase-type plasminogen activator

molecular weight :

48507 MW

protein function :

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

subcellular localization :

Secreted.

tissue specificity :

Expressed in the prostate gland and prostate cancers.

protein name :

Urokinase-type plasminogen activator

sequence similarities :

Belongs to the peptidase S1 family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1211 583 1373 211 536 1493 Standard deviation 8.77 39.06 74.14 12.87 44.48 103.01 CV (%) 4.5% 6.7% 5.4% 6.1% 8.3% 6.9%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 195 183 178 191 186 6.64 3.5% Sample 2 583 571 542 578 568 15.88 2.7% Sample 3 1373 1415 1322 1294 1351 46.55 3.4% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

normal range citations :

20369484

template :

elisa-kits

more info or order :

Boster product webpage