Human TRAIL / CD253 ELISA Kit PicoKine¬Æ | Boster EK0532 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human TRAIL / CD253 ELISA Kit PicoKine¬Æ

catalog :

EK0532

more info or order :

Boster product webpage

citations: 1

Reference
Gasowska Bodnar A, Bodnar L, Synowiec A, Jerzak M, Wcislo G, Smoter M, et al. Serum TRAIL levels in patients with epithelial ovarian cancer or primary peritoneal cancer treated with neoadjuvant chemotherapy. A pilot study. Eur J Gynaecol Oncol. 2011;32:642-6 pubmed

image

image 1 :

Human TRAIL PicoKine ELISA Kit standard curve

product information

sku :

EK0532

status :

name :

Human TRAIL / CD253 ELISA Kit PicoKine¬Æ

url key :

human-trail-picokine-trade-elisa-kit-ek0532-boster

product category :

Picokine ELISA Kits

gene name :

TNFSF10

price :

400

assay range :

15.6 pg/ml - 1,000 pg/ml

description :

Human TRAIL / CD253 ELISA Kit PicoKine (96 Tests). Quantitate Human TNFSF10 in cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 1pg/ml.

short description :

Human TRAIL / CD253 ELISA Kit PicoKine (96 Tests). Quantitate Human TNFSF10 in cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 1pg/ml.

size :

96 wells/kit, with removable strips.

uniprot id :

P50591

immunogen :

Expression system for standard: NS0; Immunogen sequence: T95-G281

storage :

Store at 4¬∞C for 6 months, at -20¬∞C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<1 pg/ml

kit components :

10 ng/tube

sample type :

cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA) and saliva.

applications :

ELISA

reactivity :

Human

images :

/e/k/ek0532_2.png

image labels :

Human TRAIL PicoKine ELISA Kit standard curve

sample data :

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.026 0.078 0.111 0.195 0.377 0.659 1.166 1.912

created at :

5/18/14 13:17

updated at :

3/2/22 2:54

meta title :

Human TRAIL / CD253 ELISA Kit Price PicoKine¬Æ BosterBio

meta keyword :

Human TRAIL ELISA Kit PicoKine, Bosterbio High Sensitivity

meta description :

Human TRAIL / CD253 ELISA Kit PicoKine (96 Tests). Quantitate Human TNFSF10 in cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 1pg/ml.

rating value :

100

anticoagulant :

heparin or EDTA

assay procedure step 1 :

1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.2 pg/ml, 15.6 pg/ml

background :

In the field of cell biology, TNF-related apoptosis-inducing ligand (TRAIL), is a protein which functions as a ligand which induces the process of cell death called apoptosis. TRAIL has also been designated CD253 (cluster of differentiation 253).Tumor necrosis factor (TNF) family cytokines function as prominent mediators of immune regulation and the inflammatory response. Most TNF family cytokines are expressed as type II transmembrane proteins, with homology confined to approximately 150 C-terminal residues. The TNF ligands interact with a parallel family of receptors. TRAIL binds to the death receptors DR4 (TRAIL-RI) and DR5 (TRAIL-RII). The process of apoptosis is caspase-8-dependent. Caspase-8 activates downstream effector caspases including procaspase-3, -6, and -7, leading to activation of specific kinases. TRAIL also binds the receptors DcR1 and DcR2, which do not contain a cytoplasmic domain (DcR1) or contain a truncated death domain (DcR2). The standard product used in this kit is recombinant human TRAIL with the molecular mass of 21KDa. It is expressed from the amino acids ectoderm T95--G28.

research category :

Apoptosis,Atherosclerosis,Cancer,Cardiovascular,Cell Biology,Cell Death,Invasion/Microenvironment,Receptors

synonyms :

Tumor necrosis factor ligand superfamily member 10;Apo-2 ligand;Apo-2L;TNF-related apoptosis-inducing ligand;Protein TRAIL;CD253;TNFSF10;APO2L, TRAIL;

gene full name :

Tumor necrosis factor ligand superfamily member 10

molecular weight :

32509 MW

protein function :

Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.

subcellular localization :

Membrane ; Single-pass type II membrane protein .

tissue specificity :

Widespread; most predominant in spleen, lung and prostate.

protein name :

Tumor necrosis factor ligand superfamily member 10

sequence similarities :

Belongs to the tumor necrosis factor family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 38 184 416 34 175 377 Standard deviation 2.66 9.56 18.72 2.75 9.62 18.85 CV (%) 7% 5.2% 4.5% 8.1% 5.5% 5%

reproducibility :

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 38 35 35 42 37 2.87 7.7% Sample 2 184 187 185 189 186 1.92 1% Sample 3 416 469 398 390 418 30.77 7.3% *number of samples for each test n=16.

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

normal range citations :

22335026

template :

elisa-kits

more info or order :

Boster product webpage